FERMNUTRAL: A POSTBIOTIC INNOVATION TO MAXIMIZE GUT HEALTH AND EFFICIENCY IN POULTRY PRODUCTION

Gut Health: The Key to Modern Poultry Production
The expression of the genetic potential of poultry largely depends on intestinal health. The gastrointestinal tract houses nearly 70% of the immune cells and constitutes the primary barrier against enteric pathogens. However, during the early stages of life, immune immaturity increases susceptibility to infections and microbiota imbalances, particularly under intensive production systems.

Traditionally, antibiotic growth promoters (AGPs) have been used to improve productive performance and reduce health-related challenges. However, their continuous use has been associated with antimicrobial resistance, intestinal dysbiosis, and concerns regarding food safety and public health.

In this context, postbiotics have emerged as a sustainable alternative. They are defined as preparations of inanimate microorganisms and/or their bioactive components, including short-chain fatty acids, bacteriocins, exopolysaccharides, peptides, and cell

wall fragments. Unlike probiotics, postbiotics offer greater stability, safety, and consistency, making them an effective alternative to AGPs (Zhong et al., 2022).

Postbiotics vs. Probiotics: A Real Competitive Advantage
The probiotics most commonly used in poultry production, such as Lactobacillus spp. and spore-forming Bacillus spp., present certain limitations when compared with postbiotics.

Lactobacillus species are heat-sensitive microorganisms; therefore, their survival during feed pelleting and storage may be compromised. In addition, factors such as chlorination of drinking water can reduce their viability and, consequently, their biological efficacy.

In contrast, Bacillus spp. exhibit high resistance to elevated temperatures due to spore formation. However, their beneficial effects may depend on successful intestinal germination and on the physiological and microbiological conditions of the host. Furthermore, these bacteria do not permanently colonize the gastrointestinal tract, meaning that their biological impact may depend on continuous administration through feed or drinking water.

Postbiotics exhibit high stability during feed processing and storage, as well as in chlorinated drinking water, because they do not rely on cell viability. Moreover, their bioactive compounds can exert their effects immediately upon ingestion, without requiring germination or intestinal colonization. This allows for a faster, more reproducible, and more consistent response (De Marco et al., 2018).

FermNutral: Bioactive Profile of a Full-Spectrum Postbiotic
FermNutral is a postbiotic developed and manufactured by CPBIO through the controlled biological fermentation of the Clostridium butyricum CPBIO3000 strain using natural raw materials. (Figure1)

The product is supplied as granules with demonstrated stability at temperatures exceeding 80°C (simulating the feed pelleting process). The recommended inclusion rate for poultry is 300–500 g/t of feed. FermNutral is produced under FAMI-QS, FDA-cGMP, and ISO 9001/14001/18001 certifications, which have been maintained since 2001.

FermNutral contains a diverse range of bioactive compounds generated during the fermentation process, including teichoic acids, lipoteichoic acids, butyricin, antimicrobial peptides, and exopolysaccharides.

Within its short-chain fatty acid (SCFA) fraction, butyric acid represents approximately 40% of the total SCFAs. Other SCFAs are present at lower concentrations, including acetic acid (21.6 mg/kg) and propionic acid (81.6 mg/kg).
The vitamin fraction includes vitamin E (13.9 IU/kg), vitamin B1 (17.2 mg/kg), vitamin B2 (5.08 mg/kg), vitamin B5 (57.3 mg/kg), vitamin B6 (98.3 mg/kg), vitamin B12 (0.34 mg/kg), folic acid (30.9 mg/kg), and vitamin K3 (1.97 mg/kg). The mineral profile provides calcium (15.2%), total phosphorus (0.76%), iron (760 mg/kg), zinc (42 mg/kg), magnesium (3,600 mg/kg), and chromium (11.67 mg/kg). Additionally, FermNutral contains 17 amino acids, including glutamic acid (1.37%), alanine (0.59%), valine (0.46%), leucine (0.50%), and lysine (0.29%). Altogether all amino acids are between 10-12% in product.

This unique combination of bioactive metabolites, nutrients, vitamins, minerals, and amino acids supports intestinal integrity, microbial balance, immune function, and productive performance in poultry.

Figure 1: A postbiotic product derived from biological fermentation of Clostridium butyricum CPBIO3000 using natural raw materials

FermNutral: Bioactive Compounds
FermNutral contains a wide range of bioactive compounds generated during the fermentation process, with butyric acid being the predominant short-chain fatty acid (SCFA), representing approximately 40% of the product. Butyrate serves as a major energy source for enterocytes and promotes epithelial renewal, mucin synthesis, and the expression of tight junction proteins. These effects contribute to maintaining intestinal barrier integrity and reducing intestinal permeability.
In addition, butyrate exhibits immunomodulatory and anti-inflammatory properties through its ability to regulate epigenetic processes, promote goblet cell differentiation, and stimulate the activation of regulatory T lymphocytes. It may also enhance intestinal serotonin release, which has been associated with the gut-brain axis and productive performance. Acetic and propionic acids complement these effects by regulating the intestinal environment and inhibiting undesirable microorganisms.

FermNutral also provides teichoic acids and lipoteichoic acids, which are recognized by Toll-like receptor 2 (TLR-2) on the intestinal mucosa. These components modulate both innate and adaptive immune responses, promoting a balance between immune defense and tolerance while helping to control intestinal inflammation (Pasquina et al., 2013; Muhammad et al., 2021).

Furthermore, FermNutral supplies butyricin (a bacteriocin), bioactive peptides, and antimicrobial peptides produced during fermentation, which contribute to the control of undesirable microorganisms and the modulation of intestinal microbiota. Exopolysaccharides are also present and have been reported to stimulate secretory IgA production and strengthen the mucosal barrier. In addition, the product’s vitamin and mineral profile acts synergistically to support immune function, antioxidant status, intestinal integrity, and productive performance.

FermNutral in poultry production
Several experiments conducted in China and Thailand have demonstrated the efficacy of FermNutral in poultry production. In a study at Guizhou University (China) comparing a control diet with additives such as FermNutral, Clostridium butyricum, protected sodium butyrate, and tributyrin in broiler chickens, the postbiotic (FermNutral) was confirmed to improve weight gain (+13%) and reduce feed conversion ratio (-11.68%) compared with the control and other experimental treatments.

In addition, another experiment carried out in a commercial broiler farm in China found that FermNutral reduced the feed conversion ratio (1.73 vs 1.77) and improved viability (+1.94) and the European Production Efficiency Factor (EPEF; 338.70 vs 315.30) in relation to the control diet.
Likewise, in a commercial broiler farm in Thailand, the use of FermNutral increased weight gain (+4.2%) and EPEF (+11.70%) and reduced feed conversion ratio (-7.69%). Furthermore, other trials conducted at Guizhou University (China) showed that FermNutral increased villus height in the duodenum (+9%), jejunum (+4%), and ileum (+8%), and improved the villus height to crypt depth ratio (V/C) in the duodenum (6.92 vs 6.27) and jejunum (5.71 vs 5.04).

Figure 3 : FermNutral effect on Immunoglobulins

Additional results from this experiment reported that FermNutral decreased the population of Escherichia coli in the duodenum (0.014 vs 0.234) and jejunum (0.0004 vs 0.013). Moreover, it promoted the growth of Lactobacillus in different intestinal segments, as determined by the relative abundance of microorganisms through 16S rDNA sequencing. (Figure 2) Finally, the inclusion of FermNutral in the diet improved the concentration of IgA (+13%), IgG (+19%), and IgM (+24%) compared with the control diet. (Figure 3)

Figure 2 : 16S rDNA sequencing to determine relative abundance of microorganisms in Poultry Intestine

Other trials conducted on a commercial farm in China with 48-week-old laying hens showed that FermNutral improved laying rate (+1.4) and shell thickness (+0.05 mm), while reducing feed conversion ratio (-0.06) and broken eggs (-2.6%), without affecting other productive performance parameters.

Likewise, in an experiment carried out at Henan Agricultural University, China, using 50-week-old laying hens, FermNutral increased egg weight (61.63 vs 59.77 vs g) and improved feed conversion ratio (2.08 vs 2.11 vs), while reducing dirty eggs (0.17 vs 0.45 %), broken eggs (0.32 vs 0.47 %), and unfit eggs (0.15 vs 0.28 %). These results demonstrate that FermNutral is an innovative and differentiated alternative within the new generation of postbiotics.

References
De Marco S, et al. (2018). Probiotic cell-free supernatants exhibit anti-inflammatory and antioxidant activity. Evidence-Based Complementary and Alternative Medicine.
Liu H, et al. (2023). Postbiotics modulate gut health and laying performance in laying hens. Animal Nutrition, 14, 244–256.
Muhammad I, et al. (2021). Lipoteichoic acid from Bacillus subtilis improves immune function of broiler chickens. Antioxidants, 10(9), 1414.
Pasquina LW, et al. (2013). Teichoic acid biosynthesis as an antibiotic target. Current Opinion in Microbiology, 16(5), 531–537.
Zhong R, et al. (2022). Gut microbiota and its interactions with postbiotics in animal production. Frontiers in Microbiology, 13, 831495.
About CPBIO – A Global Biotech Company, Based on the global practice of Charoen Pokphand Group in the agricultural, animal husbandry and food industry chain, CPBIO has extensively and deeply participated in the health cause of animals, the earth and human beings with the thinking of the whole industry chain. Organizations and individuals in large-scale validated products and solutions.

About CPBIO – A Global Biotech Company, Based on the global practice of Charoen Pokphand Group in the agricultural, animal husbandry and food industry chain, CPBIO has extensively and deeply participated in the health cause of animals, the earth and human beings with the thinking of the whole industry chain. Organizations and individuals in large-scale validated products and solutions.

By : Dr Maloshrie Bora, Program Manager (Trace Minerals), Trouw Nutrition South Asia

Trace minerals such as zinc, copper, and manganese are fundamental to poultry health, acting as cofactors in vital biochemical pathways: skeletal development, immune defenses, antioxidative systems, enzyme functions, feathering, and reproductive performance. Yet, the typical composition of feed ingredients often falls short of modern poultry standards. That’s why precision mineral nutrition—providing the right mineral at the right time and in the right form—is essential to support optimal broiler growth, eggshell integrity in layers, and fertility in breeders.

While inorganic sources like sulfates and oxides have been staples for decades, they suffer from low bioavailability and reactiveness. These soluble compounds can prematurely release minerals, which then form insoluble complexes with phytate or binding agents in the gut, diminishing absorption and even degrading vitamins or enzymes in the premix. This not only reduces feed efficiency but also increases mineral excretion, raising environmental concerns. Organic (chelate) minerals improved this situation, but often at a premium cost and with variable potency. Enter the next generation: hydroxy trace minerals. Hydroxy trace minerals, like copper, zinc, and manganese hydroxychloride, represent the latest leap in mineral nutrition. Their crystalline, covalent structure is non-hygroscopic and non-reactive in feed and the upper gut. This structure allows slow, controlled release of minerals at the ideal intestinal absorption site, significantly improving bioavailability. They resist premature dissolution, ensuring minerals are released more slowly and absorbed where it matters most.

Research across poultry sectors consistently shows that hydroxy trace minerals outperform inorganic sources. Broilers fed hydroxy copper and zinc achieved 7–8% heavier carcasses and a noticeable boost in breast meat yield. In independent trials, hydroxy-supplemented flocks maintained or improved feed conversion ratios while using lower inclusion levels than sulfate-based diets . Moreover, in antibiotic-free or necrotic enteritis challenge models, hydroxy minerals reduced pathogen load and mortality, performing on par with ionophores. Layers also benefit: eggshell quality improves, feed remains stable longer (less oxidation), and FCR gains are consistent when inorganic Cu, Zn, Mn are replaced with hydroxy versions. Breeder flocks, too, see enhanced fertility and hatchability under precision hydroxy mineral regimes.
Beyond performance, hydroxy trace minerals contribute to gut integrity and immune defense. Broilers on hydroxy mineral diets exhibited reduced cecal enterobacteria and maintained tight junction integrity, translating into healthier birds and better carcass quality.

Discover IntelliBond®: Precision You Can Trust
Among hydroxy trace mineral solutions, Trouw Nutrition’s IntelliBond® stands out as a premium, thoroughly validated choice. Designed to optimize delivery of copper, zinc, and manganese, IntelliBond features:

– High bioavailability and potency : thanks to stable, covalent crystalline bonds that release minerals at the optimal intestinal site.

– Enhanced feed stability and nutrient preservation : safeguarding enzymes like phytase and vitamins from degradation in premixes

– Improved bird performance and economics : with independent studies showing better feed conversion, heavier carcasses, superior egg output, and healthier flocks under stress.

– Environmental sustainability : with reduced inclusion rates and lower mineral excretion promoting cleaner production.

– Unmatched versatility across poultry species and life stages : including broilers, layers, and breeders—even under challenging conditions like heat stress or compromised hygiene. This adaptability has been validated across multiple trials and production environments.

Proven Performance Across Poultry Types
A Spanish study comparing hydroxy vs. sulfate-fed broilers at nutritional levels found that those receiving hydroxy minerals (IntelliBond C and Z) achieved 7.4% higher live weights, 7.7% heavier carcasses, and 16.1% breast meat yield, versus 15.3% in the sulfate group. Another Trouw Nutrition joint trial with the University of New England demonstrated improved bone integrity (tibia breaking strength) and breast meat zinc content in broilers fed 100 ppm IntelliBond Zn, with gut integrity maintained. In antibiotic-free commercial conditions, hydroxy copper-chloride combined with organic acids matched or exceeded the performance gains of feed antibiotics while improving egg weight, mass, and feed efficiency in layer hens. These findings highlight the ability of IntelliBonds to deliver consistent productivity gains across broilers, layers, and breeders—even under stress or antibiotic-free regimes. Trouw Nutrition India has been pioneering mineral-precision feeding. “Trouw Talks” events in Karnal and Hyderabad, unveiled IntelliBond’s OptiSize® technology—highlighting uniform, stable crystals that protect premix integrity and animal performance. Trouw Nutrition’s new premix plant near Hyderabad supports local production of trace minerals, vitamins, and specialized premixes—readying India for advanced feed solutions. This investment and local research infrastructure underline Trouw Nutrition’s strong commitment to validating hydroxy mineral efficacy under Indian production conditions.

Why IntelliBond® Stands Out
Developed over two decades and backed by 200+ global trials, IntelliBond® hydroxy trace minerals ensure predictable delivery and dependable results through:
– Superior bioavailability due to controlled release and crystalline stability

– Enhanced feed stability, maintaining vitamins, enzymes, and reducing oxidation in premixes

– Animal performance gains, improving carcass weight, egg production, feed conversion, and profitability

– Gut health, by reducing pathogenic bacteria and preserving gut barrier integrity in broilers
– Environmental responsibility, lowering mineral excretion while supporting sustainability-focused operations

Precision Manufacturing and Traceability
Trouw Nutrition’s OptiSize® technology guarantees uniform particle size and non-hygroscopic behavior. Its low reactivity protects feed integrity, while rigorous traceability—from raw material origins to lot distribution—ensures feed safety and compliance.

Modern poultry production demands precision: the right trace mineral, in the right form, at the right level. Hydroxy trace minerals—especially IntelliBond®—deliver on that promise. Scientific evidence and Trouw Nutrition’s local investments prove that these superior minerals enhance productivity, welfare, and sustainability in broilers, layers, and breeders. By choosing IntelliBond®, nutritionists and producers gain a trusted, research-backed solution that fosters better performance, protects investments, and advances poultry industry goals in India and beyond.