Dr Bhaskar Choudhary
Animal Nutritionist
Biochem Zusatzstoffe Handels- und Produktionsgesellschaft mbH

Abstract:
In the modern poultry industry, ensuring optimal health and productivity in layers, breeders, and broilers under various stress conditions is vital. Feed additives like choline chloride, synthetic betaine (anhydrous and HCl forms), and natural betaine are used to enhance performance. However, synthetic choline chloride and betaine often contain residues of ethylene oxide and trimethylamine (TMA), which pose significant risks to poultry health, including fatty liver syndrome, reproductive challenges, and respiratory hazards. The chemical synthesis of these additives highlights the adverse effects of residue contamination and explains why natural Betaine (anhydrous )(Hepatron/Beta Pro BL) is the superior choice.

1. Chemical Synthesis of Choline Chloride, Betaine, and Betaine Hcl
Choline Chloride Synthesis:
Choline chloride is produced by reacting ethylene oxide with trimethylamine, followed by neutralization with hydrochloric acid:
C2H4O + (CH3)3N + HCl —- (CH3)3N+CH2CH2OH.Cl-

Synthetic Betaine Anhydrous Synthesis:
Betaine is synthesized by methylating glycine with trimethylamine:
NH2CH2COOH + 3(CH3)3N—– (CH3)3N+CH2COO-

Betaine Hydrochloride Synthesis:
Betaine HCl is formed by reacting betaine with hydrochloric acid:
(CH3)3N+CH2COO- + HCl —– (CH3)3N+CH2COOH.Cl-

2. Risks Associated with Ethylene Oxide and Trimethylamine Residues
Ethylene Oxide (EO): permissible limit 0.2mg/g
Source: Ethylene oxide is used as a key reactant in choline chloride synthesis.

Risks and Effects:
Fatty Liver: Ethylene oxide residues exacerbate lipid accumulation in the liver, leading to fatty liver syndrome, impairing metabolism and egg production in layers.
Reproductive Challenges: In breeders, EO residues can induce oxidative damage to ovarian tissues, affecting fertility and hatchability.
Respiratory Hazards: Chronic exposure to ethylene oxide fumes or residues increases oxidative stress in respiratory tissues, leading to reduced lung function and increased susceptibility to respiratory infections.

Trimethylamine (TMA): permissible limit 10 mg/kg
Source: TMA is used as a methyl donor in the production of choline chloride and synthetic betaine.

Risks and Effects:
Fatty Liver: Excess TMA disrupts lipid metabolism by impairing the synthesis of very low-density lipoproteins (VLDL), leading to hepatic fat accumulation.
Reproductive Challenges: In breeders, TMA residues interfere with reproductive hormone balance, reducing fertility and chick quality.
Respiratory Hazards: Volatile TMA emissions irritate the respiratory tract, causing chronic respiratory distress in broilers and layers, especially in poorly ventilated environments.

3. Challenges of Synthetic Additives in Poultry Nutrition
Residue Toxicity: Synthetic choline chloride and betaine often leave traces of ethylene oxide and TMA, causing long-term health risks.
Liver Dysfunction: These residues impair liver detoxification and metabolic efficiency, leading to reduced productivity.
Limited Stress Resilience: Synthetic forms lack the bioactive properties of natural betaine, making them less effective in managing stress.

4. Natural Betaine (anhydrous) (Hepatron/Beta Pro BL): A Safer and More Effective Solution
Residue-Free and Safe: Hepatron/Beta Pro BL, derived from natural sources, is free of ethylene oxide and TMA residues, eliminating the associated risks of liver damage, reproductive issues, and respiratory stress.
Superior Liver Support:
– Enhances lipid metabolism, preventing fatty liver syndrome.
– Boosts detoxification pathways to handle feed-related toxins more effectively.
Enhanced Stress Management:
– Natural osmoregulatory properties stabilize cellular hydration under heat and osmotic stress.
– Promotes better feed conversion and growth performance.

5. Correlation Between Natural Betaine and Poultry Health
Fatty Liver Syndrome Prevention: Natural betaine spares choline and methionine in feed, reducing the metabolic burden on the liver and enhancing lipid transport efficiency.
Reproductive Health Support: Hepatron/BetaPro BL optimizes methylation pathways, improving ovarian function, egg production, and hatchability in breeders and layers.
Respiratory Protection: Unlike TMA-containing additives, Hepatron/Beta Pro BL improves cellular hydration and stress tolerance, protecting the respiratory tract from environmental and metabolic stress.

6. Stress in Poultry: A Multi-Faceted Challenge
Types of Stress in Poultry:
1. Environmental Stress: Heat & cold (Environment) stress in broilers & layer
2. Nutritional Stress: Imbalanced diets and mycotoxin contamination.
3. Physiological Stress: Vaccination, debeaking, and transportation.
4. Production Stress: Egg production in layers and rapid growth demands in broilers.

Role of Hepatron/Beta Pro BL in Feed application for Stress Mitigation:
Layers: Reduces egg drop during heat/Cold stress (Environment physiologica stress/ and improves shell quality.
Breeders: Enhances fertility and hatchability under environmental and nutritional stress.
Broilers: Improves growth performance and livability during transportation and heat stress.
Application of Hepatron/BetaPro BL in Drinking water: 6 hours improved water intake during treatment & outbreak condition it is advisable apart from stress mitigation what mentioned in Feed application for quick support as a clinical Nutrition

7. Why Natural Betaine (Hepatron/Beta Pro BL) is Superior

Conclusion
Residues of ethylene oxide and trimethylamine in synthetic choline chloride and betaine pose significant risks to poultry health, including fatty liver, reproductive challenges, and respiratory hazards. Natural (anhydrous )Betaine (Hepatron/Beta Pro BL) offers a safer, residue-free alternative with superior bioavailability and efficacy. By supporting liver function, improving reproductive outcomes, and protecting respiratory health, Hepatron/Beta Pro BL proves indispensable for sustainable and profitable poultry farming.
References are available on request.

Nuance Biotechnology, a rapidly emerging player in the feed additives industry, has made significant strides in 2024 by acquiring two prominent French companies—Global Nutrition International and NutriConcept. This strategic move has bolstered their global footprint, expanded R&D capabilities and facilitated their entry into key markets, including India. Poultry Creations, along with other poultry media, had the privilege of speaking with Dr. Nemanja Todorovic, Chief Business Officer and Mr. Joginder Singh Uppal, Business Director, South Asia, during the 35th AGM of the Poultry Federation of India in Gurugram to discuss their vision, innovations and plans for the Indian market

Entering the Indian Market and Expansion Strategies:

When did Nuance Biotech enter the Indian market and what are your strategies for expansion?
Dr. Nemanja Todorovic: India is not just a country; it is a continent in itself with immense potential in biotechnology and livestock. Recognizing this, we have appointed knowledgeable professionals like Mr. Uppal to lead our initiatives. Our key strategies include:
1. Forging strategic partnerships with key industry players.
2. Expanding distribution networks for broader market penetration.
3. Collaborating with research institutions to localize our products.
4. Educating farmers on the benefits of postbiotics and other innovations tailored to Indian conditions.”

Joginder Singh Uppal: “We launched our South Asian operations in June 2024 with a strong focus on poultry and dairy. Our flagship products, Nu.biom Aves for poultry and Nu.biom Bos for dairy, enhance resilience, performance and farm profitability. Our goal is to empower farmers through innovative solutions backed by scientific research and technical expertise.”

Understanding Postbiotics

Question: Can you explain the difference between postbiotics and other types of biotics?
Dr. Nemanja Todorovic: “Biotics are broadly categorized into prebiotics, probiotics, and postbiotics:
1. Prebiotics serve as a nutrient source for beneficial gut bacteria.
2. Probiotics are live microorganisms that improve gut health.
3. Postbiotics are metabolic byproducts of probiotics, offering stable and targeted benefits without the viability concerns of probiotics.

Our postbiotics enhance gut microbiota balance, optimize nutrient utilization and contribute to immune modulation. Imagine prebiotics as soil, probiotics as rice growing in the field and postbiotics as rice noodles ready for consumption.”

Tailoring Solutions for the Indian Market

Question: How does Nuance Biotech cater to the unique needs of the Indian market?
Joginder Singh Uppal: “We offer tailored solutions to address challenges in poultry and dairy farming, such as:
Postbiotics: A novel concept with proven benefits for immunity, egg quality and overall productivity.
Global Biotics: Products like mycotoxin binders (e.g., Global Fix) and acidifiers that address vital industry concerns.

Our goal is to support farmers with innovative products backed by research, while also educating them about sustainable and profitable farming practices.”

Market Strategy and Customer Engagement

Question: Who is your primary target in the market—feed mills, breeders, GP operators, commercial broiler farmers or integrators?
Joginder Singh Uppal: “Our primary focus is on feed mills since our products are predominantly feed additives. About 60-70% of the industry relies on feed, making this our initial target. Our secondary focus is integrators and large-scale farmers, including layer farmers who produce their own feed. Products like NutriGut and Globacid DW are designed for these segments.”

Unique Selling Proposition and Market Entry

Question: With several toxin binders and acidifiers in the market, what makes your products stand out?
Dr. Nemanja Todorovic: “Our precision production technology sets us apart. From raw material selection and fermentation to production and quality control, every step is meticulously executed. Our R&D-driven approach ensures high efficacy. Global Nutrition, part of our portfolio, has been a trusted name since 2002, exporting to over 40 countries. This global presence underscores the reliability and effectiveness of our products.”

Question: Have your products been introduced to the Indian market yet?
Dr. Nemanja Todorovic: “We commenced operations in mid-2024, focusing on key partnerships and collaborations. Our first consignment is scheduled to arrive next month, allowing us to engage with clients and initiate trials. Discussions with several companies are already underway.”

Future Innovations and Long-Term Vision

Question: Can you share upcoming innovations or product launches for the Indian poultry sector?
Joginder Singh Uppal: “Our focus extends beyond product launches—we provide integrated solutions. Enhancing animal resilience is at the core of our philosophy. By leveraging our expertise in microbial metabolites, we develop solutions tailored to specific market needs. While poultry and dairy remain our primary sectors, we are continuously evolving to address industry challenges.”

Question: What are your long-term plans for the Indian market?
Dr. Nemanja Todorovic: “Our strategy is structured around three key phases:
1. Establishing Partnerships – We have made significant progress in forging valuable collaborations.
2. Regional Expansion – In addition to India, we are targeting Pakistan, Bangladesh, Nepal and Sri Lanka.
3. Direct Sales and Marketing – While working with distributors for outreach, we also plan to engage directly with key accounts representing 50-60% of the poultry industry.”

Sustainability and Industry Challenges

Question: What steps is Nuance Biotech taking to ensure product sustainability?
Dr. Nemanja Todorovic: “Sustainability is at the core of our innovation strategy. We focus on enhancing nutrient utilization, reducing waste and minimizing environmental impact. For example, our solutions target ammonia control in poultry and aquaculture. Additionally, our postbiotic solutions have shown promise in reducing methane emissions in dairy cattle, and we plan to conduct further trials in India with leading research institutions.”

Question: Unlike poultry, the Indian dairy sector consists largely of unorganized small farmers. How does Nuance Biotechnology plan to support them?
Dr. Nemanja Todorovic: “About 80% of India’s dairy farmers operate at a small scale. We aim to reach them through a three-pronged strategy:
1. Strengthening our distribution network to enhance accessibility.
2. Focusing on large dairy operations that drive significant market influence.
3. Leveraging veterinary shops as a direct channel for small-scale farmers, a model that has succeeded in similar markets.”

In conclusion, Nuance Biotech is positioning itself as a pioneering force in the feed additives sector, committed to innovation, sustainability and farmer education. As they expand their presence in India and beyond, their solutions promise to enhance efficiency, profitability and resilience in the livestock industry.

ZAGRO SINGAPORE Expands Manufacturing with New Facility in India

ZAGRO Singapore, along with its subsidiary PL Agro Technologies Ltd is seen to be bringing a revolution in animal nutrition. PL Agro Technologies Ltd. India, located in Chennai was acquired by ZAGRO Singapore in 2023.

ZAGRO Singapore, a renowned leader in animal health and nutrition, is reinforcing its commitment to providing innovative and high-quality solutions across the global animal nutrition market. Through its strategic acquisition of PL Agro Technologies Ltd. in 2023, ZAGRO has solidified its presence in India, where it now operates a state-of-the-art manufacturing facility in Chennai, further enhancing its pan India footprint and product offerings.

The partnership between ZAGRO Singapore and PL Agro Technologies Ltd. marks a pivotal moment in the company’s journey, enabling it to expand its capacity for production while ensuring that the products are locally relevant and cost-effective for the Indian and global markets. At the heart of this expansion is the PL Agro facility, which now serves as the dedicated production hub for a comprehensive range of animal nutrition products designed to support the poultry industry and other animal health sectors.

The facility manufactures a variety of important products, including mineral premixes (both organic and inorganic), mycotoxin binders, Halquinol, oral liquids, Tiamulin 80% and customized products. These offerings are crafted to meet the diverse and evolving needs of the industry, providing solutions that not only enhance animal health but also ensure performance consistency and cost-efficiency.

ZAGRO Singapore’s decision to produce at the PL Agro facility is rooted in its unwavering confidence in the operational excellence of the plant, which boasts cutting-edge manufacturing capabilities and world-class quality control measures. The strategic importance of this facility is amplified by its ability to meet the increasing demand for high-quality animal nutrition solutions, all while maintaining local production standards and ensuring compliance with international regulations.
ZAGRO remains steadfast in its commitment to its core philosophy: “Global Technology, Local production and effective Solutions.” This guiding principle drives the company to deliver high-quality products tailored to local needs while adhering to global standards. Through sustainable practices and innovation, ZAGRO focuses on building long-term, reliable relationships with customers, distributors, and industry stakeholders across the globe.

The establishment of the PL Agro facility as the main production hub underscores ZAGRO’s long-term vision for India as a strategic market and a vital part of its global operations. With this move, Zagro aims to increase its market penetration, reduce product lead times, and enhance its responsiveness to customer needs.

Furthermore, ZAGRO Singapore is keen on engaging more directly with the poultry and animal health industries. The company’s goal for 2025 is to further strengthen its connections with poultry farmers, veterinarians, feed millers, and other industry professionals throughout India. This initiative aims to create stronger bonds, understand customer demands better, and offer even more tailored solutions.
For further inquiries or more detailed information, please contact:

Mr. Manas Mitra
Head of Business Operations, Zagro India
Email: manas.mitra@zagro.com

For Technical Assistance / Product Information
Mr. Raja Vadivel
R&D Project Manager, Zagro Singapore
Email: raja.vadivel@zagro.com

Visit our company websites for more information about us.
www.plagro.in
www.zagro.com

CINNAMALDEHYDE-Everything the plants have that you want- LEARNING BY DOING SCIENCE…

Deep Chand Vashishtha, – M.Sc, MBA
NSM- Bioncia International Pvt Ltd

Science is a novel practise to allow potential of ingredients or elements. It will be very helpful when it meets your requirements So well said that Learning by Doing Science…Plant bioactive compounds, such as phytochemicals, in poultry diets, are gaining popularity due to their potential antioxidant and anti-microbial activities. Phytogenic feed additives (PFAs) have emerged as natural alternatives to antibiotic growth promotors and have great potential in the poultry industry. In recent years, cinnamon (one of the most widely used spices) has attracted attention from researchers as a natural product with numerous health benefits for poultry. The essential oils in cinnamon, in particular, are of interest because of their antioxidant, anti-microbial, anti-inflammatory, antifungal, and hypocholesterolaemic effects, in addition to their ability to stimulate digestive enzymes in the gut.

Know Values of Poultry Industries
India Poultry Feed Market was valued at USD 3.27 billion in 2024 and is anticipated to project impressive growth in the forecast period with a CAGR of 6.21% through 2030.17 Sept 2024The demand for poultry feed is expected to increase due to the country’s growing poultry production, the expanding retail and food service industry, and advancements in poultry breeding techniques. However, high feed costs may put downward pressure on demand.

CINNAMALDEHYDE
Cinnamon belongs to the genus Cinnamomum (Lauraceae family) which contains more than 250–300 aromatic evergreen shrubs and plant trees However, only a few of these species have significant economic importance worldwide as a common spice including Cinnamomum zeylanicum (C. zeylanicum: True Sri Lankan cinnamon), C. cassia (Chinese cinnamon), C. burmanni (Indonesian cinnamon) and C. loureiori (Vietnamese cinnamon). The annual production of cinnamon is around 0.23 million metric tons, mainly cultivated in Indonesia, Sri Lanka, China, India, Vietnam, and Madagascar.

Phytochemistry Of Cinnamaldehyde
Phytochemicals are plant bioactive non-nutritive compounds that are usually found in small quantities They have different classes according to their structure and include phenolic compounds, phytosterols, phytoestrogens, glucosinolates, saponins, terpenoids, protease inhibitors and organo-sulfur containing compounds. They have significant antioxidant capacity to reduce and protect oxidative stress. Cinnamon consists of various bioactive compounds. Modern analytical techniques have enabled the characterization, identification, purification and quantification of individual compounds and the study of their potent biological activities. Generally, gas chromatography is applied to characterize volatile compounds while liquid chromatography for the identification of phenolic compounds. It is documented that cinnamon consists of natural antioxidant, anti-microbial and anti-inflammatory components such as volatile oils, flavonoids, curcuminoids, coumarins, tannins, alkaloids, xanthones, terpenoids, phenolics and other compounds in significant amounts. The concentration of volatile compounds in cinnamon essential oil (CNO) mainly depends upon the plant parts (leaves, bark, root, stem) from which it is extracted. About forty-one volatile compounds were identified from the bark oil of cinnamon (C. cassia) tree. Cinnamaldehyde (55% to 78%) is the main flavor compound in CNO extracted from bark while eugenol (59–78%) is the main compound in CNO that is extracted from leaves. The volatile oil is approximately 0.6–1% and 1–2% phlobatannins, calcium oxalate, starch, mucilage, and mannitol (sweet) in the bark. Moreover, Kim, et al. further investigated the cinnamon bark oil through GC-MS (gas chromatography– mass spectrometry) and identified seventeen different bioactive compounds. The major bioactive compounds of cinnamon are cinnamaldehyde, cinnamate, cinnamic acid, all of which play vital roles in various biological activities. The different essential oils that have been reported in cinnamon include trans-cinnamaldehyde, eugenol, cinnamyl acetate, L-borneol, L-bornyl acetate, β-caryophyllene, caryophyllene oxide, E-nerolidol, α-thujene, α-cubebene, terpinolene and α-terpine [19]. The LC-MS (liquid chromatography–mass spectrometry) analysis has shown that the concentrations of condensed tannins, proanthocyanidins (PAs) and epicatechin in cinnamon are 26.8%, 23.2% and 3.6%, respectively. Cinnamon has a high polyphenol content and the anthocyanidins (A and B procyanidins) are also present in cinnamon

Poultry Gut Health
Efficient immune system development and proper digestion and absorption of feed, water, and electrolyte balance in the gut leads to the development of strong gut health in poultry. The gut ecosystem plays a vital role in eliminating toxins and infectious agents from the intestinal tract of the poultry. Many factors influence the gut microbial ecosystem, including feed additives (phytobiotics, prebiotics, probiotics, feed enzymes, organic acids etc.), feed composition, genetics, heat stress, feeding practices on the poultry farm, among others. These factors exert a substantial impact on the gut microbiota and poultry health. The association between gut health and poultry performance is widely accepted with optimal health including proper physiological functions of the intestinal tract, morphological integrity, efficient immune response, developed barrier functions, energy balance, tissue metabolism, sustained inflammatory balance and sufficient microbiota to perform desired functions in the gut. The health of poultry is influenced by the structure and functionality of gut microbiota. The progression of acquisition and maturation of the intestinal microbiota throughout the growth period of the poultry has a marked impact on the modulation of physiological functions (nutrient digestion, immunity, intestinal barrier integrity etc.) to maintain gut homeostasis and development of the intestinal epithelium. These functions are essential to optimize energy use and efficiency of extraction by the poultry birds.

Impact of Cinnamon on the Digestibility of Nutrients
Improved utilization of feed improves the feed conversion ratio (FCR), body weight gain (BWG) and overall health performance of broiler chicken. The stabilization of the gut microbiota ecosystem and the stimulation of digestive enzymes secretion are the two well-accepted mechanisms that play a leading role in improving feed utilization and inhibiting the growth-depressing ailments related to metabolism and digestion. The potential impacts of CNO on the secretion of digestive enzymes from the intestinal mucosa and pancreas have been described in many poultry studies. These positive impacts had been confirmed to improve the digestibility of nutrients. Additionally, the bioactive compounds of cinnamon affect lipid metabolism by transporting the fatty acids in the digestive tract of broilers. The CNO has positive effects on the secretion of digestive enzymes and improves the digestibility of nutrients in the gut.

Supplementation of CNO in broilers diet increased the villus height (VH) in the duodenum and jejunum with associated increased villus surface area and the efficiency of absorption and digestion of nutrients. In addition, a greater VH means greater mucosal digestive enzyme activity, which ultimately improves the digestibility of nutrients.
To sum up, the use of cinnamon and its bioactive compounds as feed additives in poultry diets have potent effects on antioxidant status, immunity, nutrients availability and digestibility, enzymes secretion, mucus production, gut microbiota and overall poultry health, growth performance and productivity

Dr.Partha P. Biswas
M.Sc.,Ph.D.,F.Z.S.,F.Z.S.I.
Former Asso. Professor & H.O.D.,
Dept. of Zoology, R.K.Mission V.C.College,
Kolkata ,W.Bengal.
Senior Consultant, Aqua-Vet inputs,
Fin-O-Wing Formulations, Kolkata-700084

The chicken industry is becoming more vulnerable to environmental shifts, particularly high temperatures. Open-sided poultry species are susceptible to heat stress, negatively impacting growth and productivity. Factors determining heat stress include temperature radiation, humidity, metabolic rate, age, and duration. Modern commercial broilers are more sensitive to heat stress, making understanding and controlling environmental conditions crucial for poultry production and health. High temperatures in birds reduce antioxidant capacity, requiring food handling and expensive cooling. Understanding and controlling environmental conditions is crucial for poultry production and health.

Thermoregulatory Device in Chicken
Unlike mammals, birds do not have sweat glands, but they have developed a number of behavioral adaptations to cope with heat, including increased breathing rate, panting and raised wings. Commercial poultry prioritize high production, making broilers more sensitive to environmental stresses, and affecting meat quality and immune problems. Under conditions of heat stress, metabolic heat increases, and the animal succumbs to hyperthermia. In summary, it can be concluded that high ambient temperature outside the thermoneutral region during the production phase has a bad effect on meat production, meat quality and causes serious immune problems in broilers.

Heat Shock Proteins of Poultry Birds During Heat Stress
Heat shock proteins (HSPs) are stress proteins found in all living organisms that are activated by high environmental temperatures to protect cells from stressors such as heat. The 70 kDa heat shock proteins (HSP70) are a family of proteins known for their potential role in thermotolerance and widely regarded as cellular thermometers. Over expression of HSP70 has been observed under oxidative stress, leading to mitochondrial reactive oxygen species scavenging and pulmonary endothelial protection against bacterial toxins. They keep cells in order by synthesizing other proteins, attract immune cells and participate in protein assembly and degradation. Higher HSP expression is associated with better heat tolerance and is produced by all living organisms in high temperature environments.

Effects of Heat Stress in Poultry Birds
Reduced voluntary feed intake which affects the functionality of the entire digestive system High environmental temperatures activate the hypothalamus–pituitary axis, brain-gut axis and elevate plasma corticosterone concentrations, affecting the digestive system’s functionality.

This leads to changes in motility, flux patterns, secretory activity, content viscosity and pH Generation of ROS (reactive oxygen species) and the efficacy of the antioxidant defense system deteriorate. Overproduction of ROS in mitochondria can damage proteins, lipids, and DNA Heat stress can impair the feeding process, nutrient absorption and utilization, although water intake increases rapidly Upregulation of adipokines secretion (leptin and adiponectin) and the expression of their receptors can negatively regulate feed intake and calorie consumption thus resulting in decreased metabolic heat production The decline in trypsin, chymotrypsin and amylase (intestinal secretion) due to reduced feed intake often results in impairment of digestive functionality, nutrient digestibility Hypoperfusion and an increase in blood flow to the skin surface occur as an adaptive response of the circulatory system to stabilize blood pressure and promote heat loss It is known that heat challenge has an immune-suppressive effect.

Use of Dietary Phytochemicals to Reduce Heat Stress
Experimental studies on poultry birds suggest phytochemical ingestion may reduce heat stress effects. These phytochemicals can directly or indirectly influence genes and metabolic pathways, with stress reduction linked to antioxidant qualities.

Fig.3: The chicken’s response to being overheated. Chickens raised in high temperatures produce more reactive oxygen species and show signs of immunological inflammation in addition to consuming less food.

Mitigating Heat Stress Using Epigallocatechin-3-Gallate (EGCG), A Secondary Metabolite in Green Tea

Green tea’s most prevalent catechin, EGCG, is thought to be its most bioactive ingredient and possesses potent antioxidant properties. The primary cause of heat stress-induced oxidative stress in poultry is damage to tissues and cells, which is mostly manifested in an increase in MDA (malondialdehyde) concentration in such tissues and cells. It has been demonstrated that adding the polyphenol EGCG to broilers housed in thermoneutral environments may increase their antioxidant capacity. Acutely heat-stressed broilers may have greater antioxidant capacity and less oxidative damage in their muscles because EGCG may activate the Nrf2 signaling pathway.

Reducing Heat Stress in Broiler Chickens With Additional Ginger (Zingiber Officinale) and Onion (Allium Cepa)

Onion and its derivatives including saponins, aglycones, quercetin, cepaenes, flavonoids, organosulfurs, and phenolic compounds showed various pharmacological properties and therapeutic effects.When broilers are heat stressed, the combination of onion and ginger supplements increases the nutrition of the groups more than no supplementation.

According to research results, growth performance, carcass quality, antioxidant levels and immune system response of broilers are improved when fed 10 g of ginger and and 2.5 g of onion during heat stress. Ginger contains substances with powerful antibacterial and antioxidant properties, including chagaol, ginger diol and ginger diol. Ginger (2%) added to broilers suffering from heat stress significantly improved blood biochemical parameters and growth indicators compared to the control group.

Seeds of Black Cumin (Nigella Sativa) improve Bird’s Ability to Live in Heat-stressed Conditions

Black cumin seeds have been shown to have pharmacological and antibacterial properties and also contain drug-like compounds. The volatile oil (0.4-0.45%) contains saturated fatty acids, which include: nigellone, which is the only component of the carbonyl fraction. oil, thymoquinone (TQ), thymohydroquinone (THQ), dithymoquinone, thymol, carvacrol, α and β-pinene, d-limonene, d-citronellol, carvacrol, t-anethole, 4-terpineol and longifolin etc. Thymoquinone improves hatchability, pos-thatching performance and antioxidant activity of thermally stressed broiler embryos. Black cumin extract has been shown in trials to reduce serum MDA levels and protect against oxidative stress.

Hot Red Pepper (HRP) Reduces Heat Exhaustion in Birds

Ascorbic acid, or vitamin C, is abundant in capsaicin, a terpenoid found in HRP that helps prevent heat exhaustion in birds. Carotenoids, which are rich in vitamins E, C, and provitamin A (beta carotene), are known to have powerful antioxidant qualities that help prevent the damaging effects of free radicals and, in certain situations, oxidative stress, which can lead to cell death in broilers. Furthermore, it has been found that adding capsaicin, an active ingredient in red pepper that is present in grill feed at a dose of 50 mg/kg, can lessen the harmful effects of heat stress.

Moringa (Moringa Oleifera)helps to Survive Birds Under Heat Stress

Moringa leaves contain high levels of total polyphenols (260mg/100g), b-carotene (34mg/100g), kaempferol (34mg/100g), quercetin (100mg/100g), as well as a total antioxidant capacity of 260mg/100g. Kaempferol and quercetin are the flavonoids present in moringa leaves and possess strong antioxidants. It has been found that 0.3% incorporation of M. oleifera leaf meal improves the performance and physiological parameters of broilers and also helped the birds survive under heat stress.

THYME (THYMUS VULGAIS) Protects Chicks Against Heat Stress

The two most important bioactive compounds in this plant are carvacrol and thymol, which may be the primary source of thyme’s pharmacological actions. Thus research has identified linalool, thymol, carvacrol, gamma-terpineol, and geraniol as the primary components of thyme. Dietary thyme essential oil (150–200 mg/kg) is more effective at shielding chicks from the harmful effects of heat stress while also enhancing immunological function and development performance. One material that may be able to improve growth in broilers located in hot climates is thyme oil.

Coriander (Coriandrum Sativum) Seed in Ameliorating the Impact of Thermal Challenges

According to research, broilers under heat stress that are fed 2% coriander seed have higher feed intake, weight gain, reduced panting, and higher levels of corticosterone. The broilers’ poor intestinal absorptive capacity and shape may be connected to the rise in corticosterone levels during stress. Furthermore, according to a different study, adding 2% coriander to the diet helps broiler birds by lessening the effects of heat shock. The supplement, according to the author, benefitted broilers that were experiencing heat stress and enhanced their blood parameters, immunity, and overall performance.

Cinnamon (Cinnamomum Zeylanicum) Powder as Antioxidant in Thermally Challenged Birds

The common herbal plant, cinnamon contains different active phenolic compounds, which include flavones, catechin, isoflavones, flavonoids and other phenolics. The main bioactive constituent of cinnamon is cinnamaldehyde. The phenolic components function as antioxidants and can effectively scavenge ROS. Cinnamon supplements help in homeostasis due to the reduced pH caused by heat stress. It has also been reported that an increase in the activity of CAT, total antioxidant capacity and SOD and a decrease in the MDA when birds were placed in a thermally challenged environment during their finishing phase.

Turmeric (Curcuma Longa) for Heat-stressed Broilers

The yellowish pigments of turmeric, namely demethoxycurcumin, curcumin, and bisdemethoxycurcumin, are commonly referred to as curcumoids. Curcuminoids are an antioxidative compound found in turmeric. Researchers have shown the effects of turmeric powder supplement at 0.3 and 0.6 g/kg when administered to birds under heat stress. The superoxide radicals are neutralized, and there is an increase in the activity of SOD and CAT (ROS-removing enzymes or antioxidant enzymes ) and a decrease in MDA in broilers. The increased level in MDA indicates oxidative damage in liver of heat stressed broilers.

Conclusion
Heat stress can hurt poultry birds by making them grow slower, weakening their immune system, causing intestinal inflammation, and causing other health problems. It can also trigger oxidative process. But using natural substances called phytogenic compounds can help chickens who are raised in hot conditions.But more research is needed to understand the molecular changes made by medicinal herbs and the interactions between their active components, gut microbiota, and gut barriers. By using these approaches, we can improve chicken welfare and make poultry production more sustainable and efficient.

Prospects of Yeast Based Feed Additives in Poultry Nutrition

Dr.Partha P. Biswas M.Sc.,Ph.D.,F.Z.S.,F.Z.S.I.
Former Associate Professor & H.O.D.
Dept. of Zoology, R.K.Mission V.C.College,
Kolkata 700118,W.Bengal.
Senior Consultant, Aqua-Vet inputs,
Fin-O-Wing Formulations, Kolkata-700084

Due to the constant growth of the world’s population and the ban on the use of antibiotics, the production of livestock and poultry faces serious obstacles to meet the demand for meat, dairy products and eggs. Animal nutritionists are now forced to look for natural antibiotic substitutes because of this ban. In this regard, Saccharomyces cerevisiae (SC), or baker’s yeast, has attracted much interest in the last ten years. The use of probiotics is one of the most important ways to organically replace antibiotic growth promoters, or AGPs, in chicken feed. The live microbial culture contained in probiotics affects the intestinal microbiota and improves health by competing with the exclusion of pathogens. Two types of bacteria, Lactobacillus and Bifidobacteria, and yeast, especially SC, are the most important probiotic cultures used in poultry feed.

Enhancing Poultry Production with Yeast Products
Yeasts are eukaryotic unicellular microorganisms with a diameter of 3-40 microns. Of all the yeast species, S. cerevisiae is the facultative anaerobic fungus that has been used the most as a probiotic or prebiotic in poultry feed. Nutritional yeast has shown great ability to promote nutritional efficiency by stimulating digestive enzymes, enhancing immune responses and maximizing overall health. European countries have recognized SC as a safe additive in animal feed, and the US Food and Drug Administration has declared this yeast as generally recognized as safe (GRAS). Prebiotics such as fructo-oligosaccharides (FOS), MOS and glyco-oligosaccharides (GOS) have received much global attention and appear particularly promising. MOS are glucomannan protein complexes isolated mostly from yeast cell walls. In addition, β D-glucans are usually extracted from yeast cell walls (YCW).They are naturally active compounds that play an important role as biological response modifiers. Yeast can be added to bird feed in an amount of 5.0-10.0 g/kg feed.

Yeast Products
There are several types of yeast supplements, such as active yeast, yeast cell wall (YCW), purified cell wall components, and yeast cultures or extracts after yeast fermentation. These additives differ in appearance, composition of biologically active components and use in the production system.
In addition, yeast processing or fermentation conditions,
as well as yeast serotypes or strains, greatly affect the final product and subsequent livestock nutritional outcomes. Therefore, when choosing yeast ingredients, it is important to understand the differences between alternative products.As a feed additive for livestock and poultry breeding, active dry yeast has higher microbial activity. Dietary supplementation with heat-resistant active yeast number (>109 CFU) significantly improves the feed intake (at 50 mg i.e., 1 × 109 cfu /kg broiler diet ).

Yeast as Probiotic for Poultry
In poultry, the benefits of yeast probiotic supplements include increasing the production performance of broilers and increasing the resistance of chickens to enteric pathogens (Salmonella, Campylobacter jejuni, C. perfringens, or E. coli).In addition, yeast probiotic supplementation significantly reduces the colonization rate of Salmonellae. Yeast dietary supplements for birds and ducks can modulate the intestinal microbiota, inhibit bacterial colonization of the gastrointestinal tract.

Effects on Performance of Broilers
Yeast and its products can promote the growth of broilers, reduce morbidity and mortality. Because of their potential properties, their use in poultry feeding has numerically improved poultry performance on an industrial scale. The mechanism of action of beneficial effects of yeast includes: stimulation of brush-border disaccharidases, anti-adherence effect against pathogens and activation of non-specific immunity, binding of toxins and antagonistic effect against pathogenic organisms. The effect of YC supplementation on broiler performance is more evident during the growing period.It appears that an adaptation period is required before the effects of YC supplementation can be significant, as changes in intestinal morphology and immune responses take time.

Performance Of Layers Adding SC In Diet
It is well known that yeast derivatives have a positive effect on egg-laying activity. Addition of baker’s yeast (0.15 and 0.20%) significantly increased the egg production rate of layers compared to the control group during the 10-week experimental period. Addition of yeast culture (SC) (0.05, 0.1, 0.2%) to the diet of laying hens (18-19 weeks) shows the desired effect on body weight, FCR and egg production. With better FCR, yeast significantly (0.1, 0.2%) increases egg production and final body weight.

YC Increases P and Ca Digestibility
Supplemental YC increases P and Ca digestibility. Earlier studies also reported improved utilization of phosphorus or calcium in phosphorus-insufficient or sufficient diets of poultry when YC was supplemented. Some investigators reported that YC contains 1,400 units/kg of phyase. The improvement of P or Ca utilization could partly be attributed to phytase activity of YC.

Yeast-derived Prebiotics B-Glucans
The cell wall of baker’s yeast, or SC, has the most β-glucan. The β-glucan is a water-soluble polysaccharide composed of glucose units. It is an economic by-product of yeast, it has several health effects with its bioactive properties. However, the degradation and fermentation properties of yeast-derived β-glucan are still unknown.

Current research shows that yeast β-glucan can improve intestinal health and is one of the candidate prebiotics to stimulate intestinal health in aged chickens. Several reports indicate that b-glucans have significant immune-stimulating and modulating effects. They are able to promote immunity in broilers by activating macrophages and protect against oncogenesis.

Mannan Oligosaccharides
Mannan oligosaccharides (MOS) are mannose-containing carbohydrates found in the yeast cell wall. Because birds do not have enzymes to break down the mannan backbone, this oligosaccharide reaches the lower GI tract undigested. It is an immune system stimulant and can alter toll-like receptor (TLR) gene expression and stimulates cytokine production. Toll-like receptors (TLRs) are pathogen recognition receptors. In addition, MOS regulates the expression of cytokine genes, including interleukin-12 (IL-12) and interferon-c (IFN-c), which are important cytokines involved in enhancing the immune response of chickens (0.2% diet of broilers feed).

Effect of Yeast Supplementation on Gut Health
Numerous studies have been conducted on the use of yeast in animal diets to control intestinal health. Dietary yeast cell wall (YCW) affects how broiler chicks’ intestines look. Increased villus breadth, height, and number of goblet cells in the duodenum are observed in broilers fed YCW. When YCW was added to broiler meals, the growth phase’s FCR increased in comparison to control birds.

Immunomodulatory Functions of YC
Studies have verified that yeast culture (YC) inhibits harmful bacteria or promotes the growth of germs. The components of YC cell walls, namely β-glucans and α-mannans, have the potential to shield the mucosa by limiting pathogens from attaching to the vesicles and reducing the amount of antigen that comes into touch with them.The concentration of serum lysozyme, a non-specific immunological effector mostly released by phagocytes, was linearly enhanced by dietary YC. The duodenum of birds fed a diet supplemented with YC had greater levels of sIgA. The concentration of sIgA rose linearly with the dietary YC content. This implies that YC has the ability to increase the humoral immune system’s production of antibodies.

Limitations of Dietary Yeast Supplementation
While DYS is unquestionably a promising feed additive, its greater quantities in chicken feed have raised concerns about how its high phenolic and calcium content may negatively effect phosphorus absorption. High amounts of non-starch polysaccharides are abundant in DYS. Furthermore, a larger dietary intake of nucleic acid can decrease uric acid output in individuals with elevated DYS levels, which might result in severe metabolic abnormalities and anorexia.

FIG.1: Yeast extracts have shown promising use as an alternative to antibiotics. These extracts have found great penetration in animal feed applications due to their ability to improve the performance of poultry & other animals including fish.

Fig.2: Scanning Electron Micrograph of cells of SC

Fig.3: Main effects of live yeast on poultry birds