Introduction

Why it matters in modern poultry production
– Rapid multiplication of Eimeria can produce substantial intestinal injury within a short period.
– Subclinical coccidiosis may reduce feed efficiency, growth and flock uniformity without dramatic clinical signs.
– High oocyst contamination of litter increases exposure and repeated infection pressure.
– Effective control requires attention to parasite biology, farm management and appropriate anticoccidial programmes.

Etiology
Seven major Eimeria species are recognized in chickens. E. acervulina and E. maxima are frequently encountered, while E. tenella is particularly pathogenic and associated with severe caecal disease and haemorrhage (Yaqub et al., 2023). Species differ in preferred intestinal sites, lesion patterns and pathogenicity.

Transmission
The principal route is faecal–oral transmission. Birds ingest sporulated oocysts from contaminated litter, feed, water, equipment or the environment. Moisture, oxygen and suitable temperature favour sporulation, allowing infective oocysts to accumulate and maintain the infection cycle (Pal et al., 2023).
Life Cycle of EIMERIA

Ingestion of sporulated oocysts → release of sporozoites → epithelial invasion → multiplication → gamete formation → oocyst formation → faecal shedding → environmental sporulation.

Clinical Signs
Severity depends on Eimeria species, infective dose, bird age, immunity and environmental management. Signs include reduced feed intake, depression, ruffled feathers, drooping wings, enteritis, diarrhoea or dysentery, poor growth and increased mortality in severe cases (Pal et al., 2023).
– Reduced body-weight gain and flock uniformity
– Poor feed conversion and production performance
– Diarrhoea or blood-stained droppings in severe infections
– Dehydration, weakness and depression
– Mortality when tissue damage and blood loss are extensive
Species-specific sites

Post-Mortem Lesions
Post-mortem examination helps identify likely Eimeria species and estimate the distribution and severity of intestinal damage. Lesion patterns should be interpreted with clinical findings, litter condition, performance data and, where appropriate, laboratory diagnosis (Pal et al., 2023; Yaqub et al., 2023).

Key lesion patterns

Prevention and Control
Effective control integrates anticoccidial programmes with litter management, hygiene, stocking-density control, ventilation and sound flock management. Continuous or inappropriate use of a single anticoccidial can contribute to reduced efficacy and resistance selection; rotation, shuttle programmes, combinations and vaccination are among approaches used for longer-term control (Usman et al., 2011; Chapman, 2017; Noack et al., 2019).

Anticoccidial programmes
– Rotation changes the active ingredient or mode of action between production cycles.
– Shuttle programmes use different anticoccidial strategies during different feed phases.
– Combination programmes can broaden anticoccidial activity and may help address reduced sensitivity when appropriately designed (Chapman & Rathinam, 2022).
– Vaccination can provide controlled exposure to selected Eimeria strains and support protective immunity.

Ionophore vs Chemical

Combination programmes
Combining compatible anticoccidial activities can broaden control and reduce dependence on a single mode of action. Programme selection should consider disease pressure, previous anticoccidial history, susceptibility patterns, compatibility and label requirements (Chapman & Rathinam, 2022).

STALLEN’S DUAL APPROACH TO COCCIDIOSIS CONTROL
Stallen South Asia offers a dual approach to coccidiosis management with Manacox Plus, bringing together ionophore and chemical anticoccidial activity in one formulation. This approach is designed to support practical, structured coccidiosis-control programmes while reducing dependence on a single mode of action.

MANACOX PLUS
Manacox Plus is a dual-active anticoccidial formulation combining an ionophore and a chemical anticoccidial. It contains Monensin Sodium 8% + Nicarbazin 8% for practical coccidiosis prevention programmes in broilers.
Key Product Points

WHY A DUAL APPROACH
Using monensin sodium together with nicarbazin brings ionophore and chemical anticoccidial activity into one formulation. This can fit structured prevention programmes where the objective is effective control without unnecessary reliance on a single mode of action. Use should follow the approved label and veterinary guidance.
Practical use
– Use within a planned farm coccidiosis-control programme.
– Maintain good litter quality, ventilation, stocking density and drinker management.
– Review anticoccidial history and flock response when planning future programmes.
– Follow approved dose, withdrawal period and compatibility instructions.

References are available on request.

Biosecurity as an Investment: Why Disease Prevention delivers the Best Return on Investment (ROI) in Poultry Production

Prof. (Dr.) P.K. Shukla and Dr. Amitav Bhattacharyya
Department of Poultry Science, College of Veterinary Science and
Animal Husbandry, Mathura- 281001 (U.P.)

Introduction
Over the last five decades, the poultry industry has undergone a remarkable transformation. Scientific advances in genetics, nutrition, housing, disease control, and management have enabled producers to achieve unprecedented levels of productivity. Poultry meat and eggs have become among the most affordable and nutritious sources of animal protein, making the industry indispensable for global food and nutritional security. In India, poultry production has developed into an important agribusiness sector, generating employment for millions of people while supporting allied industries such as hatcheries, feed manufacturing, pharmaceuticals, processing, transportation, and retail.

Despite these achievements, infectious diseases remain one of the greatest constraints to sustainable poultry production. Diseases such as avian influenza, Newcastle disease, infectious bronchitis, infectious bursal disease, Marek’s disease, coccidiosis, salmonellosis, and mycoplasmosis continue to cause substantial economic losses. These diseases not only reduce flock productivity but also affect food safety, consumer confidence, international trade, and public health.

Under modern intensive production systems, disease outbreaks spread rapidly because of high stocking densities, frequent movement of birds and equipment, and increasing interactions among farms. Once pathogens establish themselves within a flock, economic losses accumulate through mortality, reduced growth, poor feed conversion, lower egg production, treatment expenses, and market disruptions. Consequently, preventing disease introduction is far more economical than managing disease after it has occurred.

Biosecurity has therefore become the foundation of modern poultry health management. It is no longer viewed merely as a collection of hygiene practices but as a comprehensive risk management strategy designed to prevent disease while maximizing productivity and profitability.

Economic Impact of Poultry Diseases
The financial consequences of poultry diseases extend far beyond mortality alone. Disease outbreaks affect every component of the production chain, from breeder farms and hatcheries to processing plants, retailers, and consumers.

Direct economic losses include increased mortality, poor weight gain, reduced egg production, inferior feed conversion efficiency, decreased hatchability, veterinary expenses, medication costs, diagnostic testing, and emergency vaccination programmes. Even mild infections that do not cause obvious mortality often reduce flock performance sufficiently to eliminate expected profit margins.

Indirect losses are frequently even greater. Disease outbreaks can result in movement restrictions, export bans, lower consumer demand, reduced processing efficiency, insurance costs, and disruption of supply chains. Highly pathogenic avian influenza has repeatedly demonstrated how a single outbreak can result in massive financial losses through depopulation, compensation payments, and trade restrictions.
Equally damaging are the hidden costs associated with subclinical infections. Chronic disease reduces flock uniformity, suppresses immune responses, increases susceptibility to secondary infections, and diminishes reproductive performance without producing dramatic clinical signs. Such losses often remain unnoticed but continuously erode farm profitability.
These economic realities highlight an important principle: disease prevention should not be regarded solely as a veterinary responsibility but as an essential business strategy that protects investments in chicks, feed, labour, housing, equipment, and marketing.

Prevention Versus Treatment: The Economics of Biosecurity
The principle that “prevention is better than cure” has special relevance in commercial poultry production. Intensive poultry systems house thousands of genetically similar birds in close proximity, allowing infectious agents to spread extremely rapidly once introduced.

By the time clinical signs become evident, pathogens have often multiplied extensively within the flock and contaminated the surrounding environment. Even if treatment successfully controls mortality, birds rarely regain their original production potential. Reduced feed efficiency, slower growth, delayed marketing age, poor egg production, and increased susceptibility to secondary infections frequently persist throughout the production cycle.
Treatment also involves considerable expenditure on antibiotics, supportive medications, laboratory investigations, veterinary consultation, and labour. These costs are incurred after production losses have already begun, thereby reducing overall profitability.
Biosecurity interrupts pathogen transmission before infection becomes established. Investments in perimeter fencing, controlled farm access, sanitation, water treatment, pest management, employee training, and visitor control substantially reduce the probability of disease introduction. Unlike treatment costs, preventive investments continue generating economic returns throughout successive production cycles by maintaining flock health and preserving production efficiency.
Numerous studies have shown that the cost of implementing effective biosecurity programmes is significantly lower than the cumulative losses associated with disease outbreaks. Consequently, biosecurity functions as a form of biological insurance that safeguards every other investment made in poultry production.

Principles of Poultry Biosecurity
Biosecurity is a science-based management system aimed at preventing the introduction, establishment, and spread of infectious agents. Effective biosecurity requires an integrated approach involving infrastructure, operational procedures, personnel behaviour, environmental sanitation, and continuous monitoring.
Three fundamental principles form the basis of poultry biosecurity:

Bio-exclusion focuses on preventing pathogens from entering the farm through controlled access, procurement of healthy chicks, vehicle disinfection, quarantine, and visitor management.

Bio-containment prevents infectious agents from spreading outside infected premises by implementing movement restrictions, proper disposal of carcasses and litter, and thorough cleaning and disinfection.

Bio-management minimizes disease pressure within the farm through vaccination, optimal nutrition, environmental management, pest control, water sanitation, litter management, and animal welfare practices.

Farm design also plays an important role in disease prevention. Poultry farms should be located away from other commercial farms, live bird markets, and migratory bird habitats. Clearly defined clean and dirty zones, all-in/all-out production systems, and adequate downtime between flocks reduce pathogen survival and transmission.
Personnel hygiene is equally critical. Farm workers should use dedicated clothing and footwear, maintain hand hygiene, comply with visitor restrictions, and receive regular biosecurity training. Similarly, feed and water must be protected from contamination through proper storage, water chlorination, and routine quality monitoring.
Rodent and insect control programmes, together with scientifically approved mortality disposal methods, further strengthen disease prevention by eliminating important sources of pathogen transmission.

Biosecurity as a Strategic Business Investment
Historically, many poultry producers regarded expenditures on sanitation, fencing, disinfection equipment, employee training, and surveillance as unavoidable operating costs. Modern poultry enterprises increasingly recognize that these expenditures are investments that protect all other farm assets.

Feed represents nearly two-thirds of total production costs. Healthy birds utilize feed more efficiently, grow faster, produce more eggs, and require fewer medications than diseased birds. Even modest improvements in feed conversion ratio can substantially increase profitability.
Strong biosecurity systems also improve market confidence. Consumers increasingly demand poultry products that are safe, traceable, and produced under high standards of animal health and welfare. Farms maintaining effective biosecurity programmes are better positioned to comply with certification schemes, quality assurance standards, and export requirements.
From a business perspective, biosecurity reduces biological risk while improving enterprise resilience. Disease outbreaks can rapidly destroy years of investment in genetics, housing, labour, and marketing. Preventive biosecurity therefore serves as a protective framework that preserves productivity, enhances competitiveness, and ensures long-term sustainability.
Modern poultry producers should therefore ask not whether they can afford to invest in biosecurity, but whether they can afford the consequences of inadequate disease prevention.

Practical Biosecurity Measures that Enhance Profitability
The success of any biosecurity programme depends on its consistent implementation at the farm level. While sophisticated infrastructure and advanced technologies can strengthen disease prevention, many highly effective biosecurity interventions are simple, practical, and relatively inexpensive. Their value lies not in high capital investment but in disciplined and continuous application.

The first line of defence is strict farm access control. Entry to poultry houses should be restricted to essential personnel, and all visitors should comply with hygiene protocols including hand sanitization, use of dedicated clothing and boots, and passage through properly maintained footbaths. Maintaining visitor records and enforcing a minimum downtime for personnel who have recently visited other poultry farms significantly reduce the likelihood of introducing infectious pathogens.
Cleaning and disinfection remain the cornerstone of disease prevention. Poultry houses, equipment, feeders, drinkers, egg trays, transport crates, and vehicles should be thoroughly cleaned to remove organic matter before applying appropriate disinfectants. Adequate downtime between production cycles allows environmental pathogen loads to decline naturally and improves the effectiveness of sanitation programmes.
Feed and water hygiene are equally important because they represent continuous sources of pathogen exposure. Feed should be procured from reliable manufacturers and stored under dry, rodent-proof conditions to prevent fungal contamination and mycotoxin development.
Drinking water should be regularly tested for microbial quality, chlorinated when necessary, and supplied through cleaned pipelines to minimize biofilm formation.
Integrated pest management also plays a critical role. Rodents, flies, beetles, mosquitoes, and wild birds serve as mechanical carriers of numerous bacterial and viral pathogens. Effective pest control, combined with scientific carcass disposal methods such as composting, rendering, or incineration, minimizes environmental contamination and pathogen persistence.
Finally, employee education is perhaps the most cost-effective biosecurity investment. Every worker should understand disease transmission pathways, recognize early clinical signs, and consistently follow standard operating procedures (SOPs). A well-trained workforce transforms biosecurity from a written protocol into a daily management culture that protects flock health and farm profitability.

Digital Transformation of Poultry Biosecurity
Rapid advances in digital technologies are revolutionizing poultry health management by shifting disease control from a reactive to a predictive approach. Precision livestock farming integrates artificial intelligence (AI), machine learning, sensor technologies, automation, and molecular diagnostics to improve disease surveillance and decision-making.

Artificial intelligence can analyse production data such as feed intake, water consumption, mortality, growth rate, and behavioural changes to detect early indicators of disease before clinical signs become apparent. Such predictive systems enable farmers to intervene promptly, thereby reducing mortality and economic losses.
The Internet of Things (IoT) has further strengthened biosecurity by enabling continuous monitoring of environmental variables including temperature, humidity, ammonia concentration, carbon dioxide, ventilation efficiency, and water quality. Automated alerts help managers respond immediately to deviations that may compromise bird health.
Computer vision systems are increasingly being used to monitor flock behaviour, feeding activity, movement patterns, and gait abnormalities. These technologies allow rapid identification of subtle behavioural changes associated with disease onset.
Molecular diagnostic tools such as polymerase chain reaction (PCR), real-time PCR, and genomic sequencing permit rapid detection of viral, bacterial, and parasitic pathogens, facilitating timely intervention. Blockchain-based traceability systems and cloud-based farm management platforms further enhance transparency, certification, and supply chain confidence.
Although digital biosecurity requires initial investment, its long-term benefits include reduced disease incidence, improved productivity, lower veterinary costs, enhanced decision-making, and increased resilience against emerging diseases.

Biosecurity Challenges and Opportunities in India
India has become one of the world’s leading producers of poultry meat and eggs, yet the country’s diverse production systems create unique biosecurity challenges. Large integrated commercial operations coexist with smallholder and backyard poultry farms, creating epidemiological interfaces that facilitate pathogen transmission.

One of the major constraints is uneven awareness regarding the economic importance of biosecurity. Large poultry companies generally maintain comprehensive disease prevention programmes, whereas many small and medium-scale producers continue to regard biosecurity as an additional expense rather than a productive investment. Financial limitations often prevent investment in essential infrastructure such as perimeter fencing, vehicle disinfection facilities, controlled farm entry, and scientific carcass disposal systems.
High poultry density in several production clusters further increases disease risk. Frequent movement of day-old chicks, feed, eggs, live birds, and farm personnel across states facilitates pathogen dissemination. Backyard poultry, live bird markets, and mixed farming systems involving ducks and other livestock also increase the risk of zoonotic disease transmission.
Despite these challenges, India possesses significant opportunities to strengthen poultry biosecurity. The country has a strong network of veterinary universities, diagnostic laboratories, research institutes, extension agencies, and progressive poultry companies capable of promoting scientific disease prevention. Government initiatives supporting livestock health, antimicrobial stewardship, digital agriculture, and the One Health approach can further accelerate adoption of comprehensive biosecurity practices.
Public-private partnerships involving industry associations, academic institutions, pharmaceutical companies, and producer organizations will be instrumental in developing practical, affordable, and scalable biosecurity solutions for Indian poultry farmers.

Biosecurity within the One Health Framework
Modern biosecurity extends beyond farm profitability to encompass animal health, public health, and environmental sustainability. The One Health concept recognizes the close interdependence of humans, animals, and ecosystems.

Effective poultry biosecurity reduces the circulation of zoonotic pathogens such as avian influenza and Salmonella, thereby protecting consumers, farm workers, and surrounding communities. Healthy flocks also require fewer therapeutic antibiotics, contributing to global efforts to combat antimicrobial resistance (AMR).
Good biosecurity practices improve waste management, reduce contamination of soil and water resources, and minimize environmental dissemination of infectious agents. Consequently, investments in poultry biosecurity generate societal benefits that extend far beyond individual farms by supporting food safety, environmental protection, and national food security.

Capacity Building and Policy Support
Infrastructure alone cannot guarantee successful biosecurity. Human behaviour remains one of the most critical determinants of disease prevention. Continuous training programmes should therefore be provided for farm owners, managers, veterinarians, technicians, and workers on disease recognition, personal hygiene, vaccination, cleaning and disinfection, pest control, mortality management, and emergency response.
Routine biosecurity audits, risk assessments, and comprehensive record-keeping systems enable producers to monitor flock performance and identify weaknesses requiring corrective action. Standard operating procedures should be regularly reviewed and updated in response to emerging disease threats and technological developments.
Government agencies also have an essential role in strengthening national biosecurity through veterinary services, laboratory networks, surveillance systems, science-based regulations, and financial incentives that encourage producers to invest in preventive infrastructure. Harmonization of national standards with international guidelines will further improve India’s competitiveness in global poultry trade.

Way Forward
Biosecurity has evolved from a routine veterinary recommendation into one of the most valuable strategic investments in modern poultry production. Scientific evidence consistently demonstrates that prevention delivers substantially greater economic returns than treatment. Every investment made in disease prevention safeguards valuable biological assets, preserves productivity, reduces veterinary expenditure, minimizes antimicrobial use, and enhances market confidence.
For India, where poultry production continues to expand rapidly, widespread adoption of comprehensive biosecurity systems will be fundamental to sustainable growth. Success will require coordinated efforts involving producers, veterinarians, researchers, policymakers, industry organizations, and technology providers working within a One Health framework.
The future of poultry farming will increasingly depend on predictive health management supported by digital technologies, precision farming, artificial intelligence, and robust surveillance systems. Producers who integrate biosecurity into their core business strategy will be better equipped to withstand emerging disease threats, satisfy evolving consumer expectations, and compete successfully in international markets.
Ultimately, the most profitable poultry enterprise is not the one that spends the least on disease prevention, but the one that invests wisely to ensure disease never compromises productivity. Biosecurity should therefore be regarded not as an operational expense but as a high-return investment that protects animal health, public health, environmental sustainability, and the long-term resilience of the poultry industry.

Selected References
1. Alexander, D. J. (2007). An overview of the epidemiology of avian influenza. Vaccine, 25, 5637–5644.
2. FAO. (2023). Good Practices for Biosecurity in the Poultry Sector. Food and Agriculture Organization of the United Nations.
3. Gelaude, P., Schlepers, M., Verlinden, M., et al. (2014). Measuring on-farm poultry biosecurity using Biocheck.UGent. Poultry Science, 93, 2740–2750.
4. Glisson, J. R., Hofacre, C. L., & Christensen, J. P. (2019). Poultry Diseases. Elsevier.
5. WOAH. (2023). Terrestrial Animal Health Code.
6. Swayne, D. E. (Ed.). (2020). Diseases of Poultry (14th ed.). Wiley-Blackwell.
7. World Health Organization. (2022). One Health Joint Plan of Action (2022–2026).
8. World Bank. (2022). One Health Operational Framework for Strengthening Animal Health Systems.
9. FAO. (2022). Biosecurity Guide for Poultry Production and Value Chains.
10. USDA APHIS. (2023). Defend the Flock: Biosecurity for Poultry Owners.

Introduction
Inclusion Body Hepatitis (IBH) is an economically important viral disease of broiler chickens characterized by sudden mortality, hepatitis, and the presence of intranuclear inclusion bodies in liver cells. The disease is primarily caused by Fowl Adenoviruses (FAdVs), particularly serotypes belonging to species D and E. IBH has become increasingly significant worldwide due to its impact on broiler performance, increased mortality, poor feed conversion, and substantial economic losses. The disease mainly affects young broiler chickens between 3 and 7 weeks of age, although birds of other ages may also be affected. Vertical transmission from breeder flocks and horizontal spread through contaminated litter, water, equipment, and personnel play major roles in disease dissemination.

Etiology
IBH is caused by Fowl Adenoviruses (FAdVs), non-enveloped double-stranded DNA viruses belonging to the genus Aviadenovirus within the family Adenoviridae. Several serotypes have been associated with disease outbreaks, including FAdV-2, FAdV-8a, FAdV-8b, and FAdV-11.
Predisposing factors include:
– Immunosuppression caused by Infectious Bursal Disease (IBD)
– Chicken Infectious Anemia (CIA)
– Mycotoxicosis (Poor quality of feed ingredients contaminated with fungus and Mycotoxins).
– Poor Biosecurity and Management
– High stocking density

Clinical Signs
Affected broiler flocks may exhibit:
– Sudden increase in mortality
– Depression and lethargy
– Huddling with Ruffled feathers
– Reduced feed and water consumption
– Usually affects birds of 3 to 5 weeks age, but can be seen in the chicks aging from 4-5 day old.
– Pale comb and wattles
– Poor weight gain
– Diarrhoea with mucoid droppings or green droppings
– Anemia in severe cases
Mortality rates generally range from 5–15%, but outbreaks may occasionally result in mortality exceeding 30%.

Post-Mortem Lesions
The most characteristic lesions are observed in the liver.
Gross Lesions
Liver
– Enlarged, swollen, and pale liver
– Yellowish discoloration
– Multifocal necrotic foci
– Petechial and ecchymotic hemorrhages
– Friable consistency
Kidneys
– Enlarged and pale kidneys
– Congestion
Heart
– Hydropericardium may occasionally be present
– Pale myocardium
Spleen
– Enlargement and congestion
Histopathological Lesions
Microscopic examination reveals:
– Severe hepatic necrosis
– Basophilic intranuclear inclusion bodies in hepatocytes
– Degeneration and destruction of liver cells
– Mononuclear cell infiltration
The presence of characteristic intranuclear inclusion bodies is considered pathognomonic for IBH.

Serology
Serological testing is useful for monitoring flock exposure and breeder immunity.
Common serological methods include:
ELISA (Enzyme-Linked Immunosorbent Assay)
– Detects antibodies against Fowl Adenovirus
– Useful for flock monitoring
– Evaluates maternal antibody levels
Virus Neutralization Test (VNT)
– Determines serotype-specific antibodies
– Primarily used in research and epidemiological investigations
Interpretation
– High antibody titers in breeders provide maternal protection to progeny.
– Seroconversion in broilers indicates field exposure.
– Paired serum samples can help determine recent infection.
Diagnosis
Diagnosis should be based on a combination of clinical history, post-mortem findings, histopathology, and laboratory confirmation.
Field Diagnosis
Suspect IBH when:
– Sudden mortality occurs in 3–7 week-old broilers.
– Enlarged, pale, hemorrhagic liver is observed.
– There is a history of immunosuppressive diseases.
Laboratory Diagnosis
Histopathology
– Demonstration of intranuclear inclusion bodies in hepatocytes.
Polymerase Chain Reaction (PCR)
– Highly sensitive and specific.
– Detects and identifies FAdV serotypes.
Virus Isolation
– Performed in chicken embryo liver cells or embryonated eggs.
Immunohistochemistry
– Detects adenoviral antigen within tissues.
Sequencing
– Used for epidemiological studies and strain characterization.

Treatment
There is no specific antiviral treatment for Inclusion Body Hepatitis.

Management is mainly supportive:
Supportive Therapy
– Multivitamin supplementation
– Vitamin E and Selenium administration
– Use D’Bio-Mix 20-25 mL per 100 birds and ABES-URZZA 20 ml for 100 birds.
– Ionic electrolyte (ABESTRAL) supplementation and probiotic prebiotic supplementation
— Adequate hydration
Control of Secondary Infections
– Antibiotics may be administered under veterinary supervision to control secondary bacterial infections.
Management Measures
– Reduce stress factors
– Improve ventilation
– Maintain litter quality
– Ensure proper nutrition

Prevention and Control
Effective prevention relies on biosecurity and breeder vaccination.

Biosecurity Measures
– All-in/all-out management
– Thorough cleaning and disinfection
– Restriction of farm visitors
– Proper disposal of dead birds
– Control of rodents and insects
– Sanitation of equipment and vehicles
Breeder Flock Management
– Monitor antibody levels regularly.
– Vaccinate breeders to ensure maternal antibody transfer.
– Prevent immunosuppressive diseases such as IBD and CIA.
Control of Vertical Transmission
– Maintain healthy breeder flocks.
– Conduct routine serological surveillance.
– Implement strict hatchery hygiene.

Vaccination Schedule
Vaccination strategies vary according to local epidemiology and circulating serotypes.
Breeder Vaccination Program
Age Vaccine
8–10 weeks Killed IBH (primary dose)
14–16 weeks Booster dose
18–20 weeks (before lay) Optional booster in high-risk areas
Broiler Vaccination
Routine vaccination of broilers is generally not practiced in many regions because protection is primarily achieved through maternal antibodies. However, live or inactivated vaccines may be considered in endemic areas under veterinary guidance.
Objectives of Vaccination
– Prevent vertical transmission
– Enhance maternal antibody levels
– Reduce mortality and production losses
– Improve flock uniformity

Economic Impact
IBH can result in:
– Increased mortality
– Poor growth performance
– Reduced feed efficiency
– Higher medication costs
– Increased carcass condemnation
– Significant financial losses to poultry producers

Conclusion
Inclusion Body Hepatitis remains an important viral disease of modern broiler production. Early diagnosis, strict biosecurity, breeder vaccination, and effective management practices are essential for disease prevention and control. Maintaining strong maternal immunity through breeder vaccination and minimizing immunosuppressive conditions can significantly reduce the impact of IBH on commercial poultry operations.

Weather fluctuations, particularly during seasonal transitions, create significant stress in poultry production systems. Sudden changes in temperature, humidity, ventilation patterns, and air quality weaken the birds’ immune defenses and predispose them to respiratory infections. Among these, Mycoplasmosis is one of the most economically important diseases affecting both commercial layers and breeder flocks.

The two major pathogenic species affecting poultry are:
– Mycoplasma gallisepticum infection (MG)
– Mycoplasma synoviae infection (MS)
These organisms are highly contagious and often become more problematic during changing weather conditions due to increased environmental stress and compromised flock immunity.

Why Changing Weather Increases Mycoplasma Problems
During weather transitions such as:
– Summer to monsoon
– Monsoon to winter
– Sudden cold or heat waves
birds experience physiological stress that suppresses their immune response. Several environmental factors contribute to Mycoplasma outbreaks:
1. Temperature Fluctuation
Rapid day-night temperature variation stresses birds and damages the respiratory mucosa, making it easier for Mycoplasma organisms to colonize the respiratory tract.
2. Poor Ventilation
Farmers often reduce ventilation during cold weather to conserve heat. This increases:
– Ammonia levels
– Dust accumulation
– Humidity
– Carbon dioxide concentration
Such conditions irritate the respiratory tract and favor Mycoplasma multiplication.
3. High Humidity and Wet Litter
During monsoon seasons, excessive moisture increases pathogen survival and worsens respiratory stress.
4. Secondary Infections
Changing weather also favors bacterial and viral co-infections such as:
– Newcastle disease
– Infectious bronchitis
– Escherichia coli infection

These infections intensify the severity of Mycoplasma disease.
Clinical Signs in Layers and Breeders
Affected birds may show:
– Coughing and sneezing
– Nasal discharge
– Rales and respiratory sounds
– Swollen sinuses
– Watery eyes
– Reduced feed intake
– Depression and poor growth
– Increased mortality in severe cases

In breeder flocks, fertility and hatchability may decline significantly.

Impact on Layer Performance
Mycoplasma infection severely affects commercial egg production.
1. Drop in Egg Production
Layers infected with MG or MS commonly show:
-Sudden reduction in egg production
– Irregular laying patterns
– Delayed peak production
Production losses may range from 5–20% depending on flock condition and secondary infections.
2. Poor Egg Quality
Infected layers may produce:
– Thin-shelled eggs
– Misshapen eggs
– Smaller eggs
– Poor shell strength
This increases breakage and market rejection.
3. Increased Feed Conversion Ratio (FCR)
Respiratory stress reduces feed efficiency, increasing production cost per egg.
4. Increased Culling and Mortality
Chronically affected birds become poor performers and require early culling.

Impact on Breeder Performance
The economic effect of Mycoplasma is even more serious in breeder operations.
1. Reduced Fertility
Mycoplasma infection negatively affects reproductive performance, lowering fertility percentage.
2. Poor Hatchability
Vertical transmission through eggs can infect embryos, causing:
– Embryonic mortality
– Weak chicks
– Poor hatchability
3. Transmission to Progeny
MG and MS can spread from infected breeders to chicks through eggs, creating long-term farm problems.
4. Reduced Chick Quality
Chicks from infected breeder flocks often show:
– Weakness
– Respiratory distress
– Poor growth potential
– Higher early mortality

Economic Losses
Mycoplasma outbreaks during changing weather can lead to major financial losses through:
– Reduced egg production
– Poor hatchability
– Increased medication costs
– Mortality and culling
– Lower chick quality
– Increased feed costs
– Trade restrictions in breeder operations

Diagnosis
Accurate diagnosis is essential for effective control. Common diagnostic methods include:
– Serological testing (ELISA, SPA)
– PCR testing
– Culture and isolation
– Postmortem examination

Typical lesions include:
– Air sacculitis
– Tracheitis
– Foamy air sacs
– Pneumonia

Prevention and Control Measures
1. Maintain Proper Ventilation
Ensure adequate airflow without creating drafts.
2. Reduce Environmental Stress
– Maintain uniform temperature
– Avoid sudden temperature changes
w Control humidity levels
3. Strengthen Biosecurity
– Restrict visitor movement
– Disinfect equipment
– Control wild birds and rodents
4. Vaccination
Vaccination programs against MG and MS should be followed according to veterinary recommendations.
5. Early Medication
Prompt treatment using appropriate antimicrobials under veterinary guidance can reduce severity.
6. Improve Litter and Air Quality
Regular litter management helps reduce ammonia and dust.
7. Nutritional Support
Provide vitamins, electrolytes, and immune boosters during stressful weather periods.

Conclusion
Changing weather conditions significantly increase the risk and severity of Mycoplasma infections in poultry farms. Layers suffer from reduced egg production and poor egg quality, while breeders face severe reproductive and hatchability losses. Because Mycoplasma infections often become chronic and difficult to eliminate, prevention through good management, ventilation, biosecurity, and stress reduction remains the most effective strategy.

Early detection and timely intervention are essential to maintain flock health, productivity, and profitability during seasonal

By: Dr. Priyanka Kamble, Senior Marketing Manager Huvepharma

Introduction

Newcastle Disease (ND), caused by Avian Paramyxovirus Type-1 (APMV-1), remains one of the most devastating viral infections affecting the poultry industry in India. With high mortality rates, reduced egg production, and severe economic losses, ND poses a constant threat to both small-scale poultry farmers and large commercial producers. Despite advancements in vaccination and biosecurity, the disease continues to challenge the sustainability of India’s poultry sector, which contributes significantly to the nation’s agricultural GDP.

Newcastle Disease: A Persistent Menace

Newcastle Disease is highly contagious, affecting chickens, turkeys, and other avian species. The virus spreads through direct contact, contaminated feed, water, equipment, and even airborne transmission. Clinical signs vary depending on the strain but commonly include:

• Respiratory distress (gasping, coughing, nasal discharge)
• Nervous signs (twisting of the neck, paralysis, tremors)
• Greenish diarrhoea
• Sudden drop in egg production (thin-shelled or shell-less eggs)
• High mortality (up to 100% in unvaccinated flocks)

In India, velogenic strains (highly virulent) are predominant, causing severe outbreaks that cripple poultry operations. (APMV-1 Velogenic NDV is responsible for Velogenic Viscerotropic ND (VVND) outbreaks in India).

Economic Impact on the Indian Poultry Industry

India is the third-largest egg producer and fifth-largest poultry meat producer globally, The poultry sector in India, valued at more than USD 28 billion in 2021-22, has been a vital component of the country’s agriculture and food processing industry. Newcastle Disease disrupts this growth through:

1. Direct Losses Due to Mortality & Culling

• Unvaccinated or poorly managed flocks face mortality rates of 80-100%, leading to massive financial losses.
• Government-mandated culling during outbreaks further exacerbates losses.

2. Reduced Egg & Meat Production

• Layers: A single ND outbreak can cause a 20–50% drop in egg productionand reduce egg quality, with recovery taking weeks.

• Broilers: Cause severe mortality. Infected birds suffer stunted growth, leading to lower market weights and downgrading at processing plants.

3. Increased Vaccination & Treatment Costs

• Farmers must invest in regular vaccination schedules (Live & Inactivated ND vaccines), adding to operational costs.
• Secondary bacterial infections (E. coli, Mycoplasma) increase antibiotic usage, raising concerns over antimicrobial resistance (AMR).

4. Trade Restrictions & Market Losses

• ND outbreaks lead to quarantine zones, restricting movement of poultry and products.
• Export markets (Middle East, Southeast Asia) impose bans on Indian poultry products during outbreaks, causing revenue losses.

5. Impact on Small & Marginal Farmers

• Over 70% of Indian poultry farmers are small-scale, lacking resources for strict biosecurity.
• A single ND outbreak can bankrupt small farmers, pushing them out of the industry.

Strategies to Combat Newcastle Disease

1. Strict Vaccination Protocols
2. Enhanced Biosecurity Measures

• Farm-level hygiene: Disinfection of footwear, vehicles, equipment.
• Restricted access: Prevent contact with wild birds & other farms.
• All-in-all-out systems: Reduce viral persistence in multi-age flocks.

3. Early Detection & Rapid Response

• Regular serological monitoring (HI tests for antibody titers).
• Rapid reporting of suspected cases to Veterinarians.

4. Proactive Measures for ND Outbreak Prevention

• Compulsory ND vaccination programs in high-risk zones.
• Farmer awareness campaigns on biosecurity best practices.

Conclusion: A Call to Action

Newcastle Disease is not just a health issue—it’s an economic catastrophe for India’s poultry industry. With the sector growing at 8-10% annually, unchecked ND outbreaks disrupt livelihoods and threaten national food security.

The solution lies in:
 Proactive vaccination
 Robust biosecurity
 Farmer education
 Stronger policy enforcement

As veterinarians, researchers, and industry leaders, we must unite to safeguard Indian poultry from Newcastle Disease—ensuring sustainability for farmers and safe, affordable protein for millions.

Breathing Trouble: A Glimpse into the World of CRD in Poultry
India ranks second globally in egg production and fifth in poultry meat production. The Indian poultry market, despite being one of the largest globally, remains a developing sector due to its fragmented infrastructure, inconsistent biosecurity standards, and varying degrees of modernization across regions.

A significant portion of poultry production still relies on open housing systems, limited automation, and minimal veterinary oversight, especially among smallholder and backyard farmers. These conditions foster high disease prevalence, as poor sanitation, overcrowding, and lack of structured vaccination programs create ideal environments for the spread of infectious agents like Mycoplasma gallisepticum, E. coli, and coccidia. Consequently, the industry faces substantial economic losses through reduced productivity, higher mortality, increased medication costs, and trade restrictions. Bridging the gap between traditional practices and scientific poultry management is critical for improving flock health and sustaining long-term growth.

One Breath at a Time: Poultry Farmers Battle Chronic Respiratory Disease

Before any effective fight against Chronic Respiratory Disease (CRD) can begin, the poultry industry must first understand the enemy it faces. CRD is not just another seasonal illness—it’s a complex, persistent infection primarily caused by Mycoplasma gallisepticum, capable of silently spreading through flocks and leaving devastating economic consequences in its wake. Its symptoms often mimic those of other respiratory illnesses, making early detection a challenge. Without a clear understanding of its pathogenesis, transmission, and triggers, efforts to control CRD remain reactive and insufficient. Knowledge is the first line of defense—only with education, diagnosis, and structured prevention can farmers hope to break the cycle of recurring outbreaks. The battle against CRD must begin with awareness and be fought with science, vigilance, and unity across the industry.

Unmasking the Culprit: The Hidden Cause of CRD in Poultry

CRD is caused by Mycoplasma gallisepticum (MG), a wall-less bacterium that affects the respiratory tract of poultry. Secondary infections with Escherichia coli, Ornithobacterium rhinotracheale, and viral pathogens (NDV, IBV) often exacerbate disease severity.

Silent Spread: How CRD Continues to Lurk in Poultry Farms
CRD in poultry, caused by Mycoplasma gallisepticum, spreads through both horizontal and vertical transmission. Infected birds release the pathogen via respiratory secretions, contaminating air, water, feed, and equipment. Vertical transmission from breeder hens to chicks via eggs further fuels early infection. Recovered birds often remain silent carriers, shedding the organism under stress. This makes CRD hard to eradicate and highlights the need for strong biosecurity, breeder screening, and flock management to control its spread.

How CRD Takes Hold: Understanding the Disease’s Journey in Poultry
The pathogenesis of Chronic Respiratory Disease (CRD) in poultry begins when birds inhale aerosolized Mycoplasma gallisepticum, the primary causative agent. The pathogen adheres to the ciliated epithelial cells lining the upper respiratory tract, disrupting the mucociliary clearance mechanism. This allows the bacteria to colonize and multiply, triggering a chronic inflammatory response that leads to thick mucus secretion, tracheitis, and air-sacculitis. The damaged respiratory lining also becomes highly susceptible to secondary bacterial infections, particularly from E. coli, compounding respiratory distress and systemic illness.

In commercial poultry, stress factors such as poor ventilation, high stocking density, and concurrent viral infections (like IBV or NDV) can further exacerbate disease progression, resulting in reduced growth rates, poor feed conversion, decreased egg production, and increased mortality.

Signs & Symptoms with Postmortem (PM) Findings
CRD in poultry typically presents with a range of respiratory signs that can vary in severity based on age, immune status, and presence of co-infections. Common clinical signs include coughing, sneezing, nasal discharge, tracheal rales, conjunctivitis, reduced feed intake, stunted growth, and a noticeable drop in egg production in layers. Birds may also exhibit open-mouth breathing and watery eyes. In chronic stages, swelling of infraorbital sinuses and air-sacculitis becomes evident. On postmortem examination, the most consistent findings include thickened, cloudy air sacs (airsacculitis), catarrhal to caseous exudate in the trachea and bronchi, perihepatitis, pericarditis, and fibrinous pneumonia. In cases complicated by secondary infections like E. coli, lesions become more severe, showing a classic “CRD complex.”

Integrated Strategy to Fight CRD
An integrated CRD control strategy combines biosecurity, vaccination, early detection, nutritional support, and precision medication.

Preventive Phase: Reducing the Latent Load
Forlutin 10% (Tiamulin 10%) a high-quality feed additive by Stallen South Asia Pvt. Ltd. serves as the cornerstone for preventive management. Administering it to growers between 7 to 14 weeks of age or just before expected stress periods such as vaccination or peak lay helps reduce the latent load of Mycoplasma. This approach prepares the flock by lowering the pathogen load before the birds reach a vulnerable stage.

Outbreak Management: When Clinical Signs Appear
At the onset of clinical signs indicative of Mycoplasmosis, immediate action is required. Stalmicosin (Tilmicosin Phosphate 250mg) oral solution – a high-quality product manufactured by Stallen South Asia Pvt. Ltd. in its own manufacturing facility to ensure the highest Quality standards, administered via drinking water at 15–20 mg/kg body weight, is highly effective due to its deep lung penetration and prolonged action. This should be continued for 3 to 5 days but not exceeded.

Following the Stalmicosin course, a 24–48hour break should be observed before beginning treatment with Forlutin 80% (Tiamulin 80%) water soluble powder. A dosage of 25–50 mg/kg body weight for another 3 to 5 days helps eliminate residual Mycoplasma and prevents recurrence. Integrating these antimicrobials into a scheduled rotation can significantly reduce disease recurrence and resistance development.

Monitoring and Biosecurity: Supporting the Antimicrobial Strategy
Surveillance using PCR and ELISA tests at regular intervals is vital to detect Mycoplasma presence, especially during and after stress periods. Swab sampling and necropsy examinations for lesions such as air sacculitis or swollen joints provide further evidence. Strict biosecurity—enforcing all-in/all-out practices, staff segregation, and regular disinfection using NADCC, quaternary ammonium compounds, or glutaraldehyde—is essential to support the medical interventions.

References
1. Indian Journal of Veterinary Science & Poultry Health, 2023. Comparative Efficacy of Antibiotics in CRD.
2. Practical Poultry Guide, Vol 18, 2024 – Antimicrobial Resistance Trends in Poultry Pathogens.
4. McOrist et al. (2002) – Tilmicosin pharmacokinetics and tissue distribution in avian models.
5. Poultry Science Journal, 2022 – Mycoplasma Control Strategies in South Asia.

Biosecurity Measures – The First Line of Defence Against Bird Flu

Dr. Sagrika Bhat1, Dr. Sundus Gazal2, Dr. Sabahat Gazal3and Dr. Anvesha Bhan4
1Division of Veterinary Biochemistry, 2,3,4Division of Veterinary Microbiology
and Immunology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu

Microscopic pathogens, including bacteria, viruses, fungi, and parasites, pose significant threats to poultry health, with avian influenza being a major concern due to its high mortality, economic impact, and zoonotic potential. The disease is caused by Influenza A virus belonging to the family Orthomyxoviridae. Influenza A viruses are divided into subtypes based on two proteins on the surface of the virus: hemagglutinin (H) and neuraminidase (N). There are 18 different hemagglutinin subtypes and 11 different neuraminidase subtypes (H1 through H18 and N1 through N11, respectively). The highly pathogenic strains such as H5N1, H7N9, and H9N2 have been reported to cause severe disease. The virus spreads through direct contact with infected birds, contaminated feed, water, and fomites, while wild migratory birds serve as natural reservoirs, enabling global transmission. Highly pathogenic avian influenza can lead to near-total flock mortality, significantly disrupting poultry production and trade. Additionally, zoonotic strains such as H5N1 and H7N9 can cause severe respiratory illness, pneumonia, multi-organ failure, and high fatality rates in humans, necessitating global surveillance by organizations like the World Health Organization (WHO).

Poultry farms constantly face the risk of Avian influenza and other infectious diseases that persist in dust, droppings, and farm waste, making biosecurity a fundamental component of disease prevention. Biosecurity measures serve as the first line of defence, preventing pathogen entry and transmission through stringent hygiene, controlled farm access, and optimized housing conditions. Effective biosecurity minimizes outbreaks of avian influenza, Newcastle disease, duck plague, and bacterial infections such as fowl cholera and mycoplasmosis, which compromise poultry health, reduce productivity, and weaken consumer confidence.

Given the increasing incidence of avian influenza worldwide, including India, strengthening biosecurity is imperative to safeguard poultry health and public safety. Disease prevention strategies must integrate high-quality stock, proper housing, clean feed and water, regular disinfection, and restricted farm access. Additionally, modifying industry practices in poultry production, transport, and marketing is essential to curb disease spread. Veterinary authorities must continuously evaluate and refine biosecurity measures in high-risk areas while considering economic and social impacts. Several biosecurity measures have been implemented or require further revision in Asian countries, including India, to effectively control avian influenza and ensure sustainable poultry production. Above all, biosecurity must be a continuous effort rather than a reactive response to outbreaks.

A well-structured, proactive approach remains critical for preventing disease outbreaks, ensuring industry stability, and minimizing zoonotic risks.

Key Biosecurity Measures in the Poultry Industry
1. Marketing Systems: Live bird markets serve as critical points for avian influenza (AI) transmission due to continuous operation, overnight poultry retention, and the reintroduction of unsold birds to farms. These practices facilitate pathogen circulation. Implementing a mandatory market rest period of 24 hours in a week, accompanied by thorough cleaning and disinfection, is essential to mitigate viral persistence and spread.

2. Species Segregation: Domestic waterfowl and quail act as reservoirs for avian influenza viruses. Their cohabitation, transportation, and marketing alongside other poultry should be restricted to minimize interspecies transmission. Additionally, swine reared in proximity to infected poultry farms are found to be infected with HPAI (Highly Pathogenic Avian Influenza) therefore should undergo systematic veterinary surveillance. In cases of confirmed avian influenza infection, culling of affected herds is recommended to prevent viral reassortment and potential zoonotic spillover.

3. Farming Practices: Extensive poultry rearing systems, particularly in village settings, pose a heightened risk for avian influenza introduction due to their lack of biosecurity controls. Strategic vaccination programs targeting backyard poultry can enhance herd immunity. Commercial farms should adhere to an ‘all-in, all-out’ production model to reduce pathogen exposure and poultry workers must adhere to strict biosecurity protocols, including cleaning, disinfecting, or changing protective clothing, equipment, and footwear before entering and after leaving farms.

4. Transport Biosecurity: Transport cages and egg containers should be constructed from non-porous materials such as plastic or metal over wooden cages to facilitate effective disinfection. To prevent environmental contamination and disease spread, bio-secure transport protocols should be implemented. This includes minimizing faecal contamination during poultry unloading, ensuring transport cages are cleaned and disinfected before returning to farms, and using easily sanitized materials for transporting table eggs, fertile eggs, and day-old chicks.

5. Compartmentalization: In regions where avian influenza is endemic, creating compartmentalized poultry populations with distinct health statuses is essential for disease control and international trade compliance. This requires strict biosecurity measures, including traceability of fertilized eggs, certified hatchery and feed sources, vermin control, and regulated transport. Poultry operators must maintain detailed records of suppliers, egg crate circulation, employee responsibilities, and transport activities to ensure compliance and effective disease containment.

Mitigation of Stress through Managemental Interventions
While biosecurity is crucial for disease prevention, stress reduction is equally important in enhancing poultry resistance to infections, including avian influenza. Environmental factors such as high temperatures, ammonia build-up, overcrowding, feed deprivation, handling, and transportation induce physiological stress, compromising immunity. Strategies such as adjusting feeding schedules, providing cool drinking water, supplementing essential nutrients, and optimizing dietary energy and amino acid levels help mitigate heat stress. Maintaining appropriate temperature, ventilation, and humidity is vital for flock health, especially in regions with high heat and humidity. Since wet litter contributes significantly to ammonia production, proper litter management, ventilation, and dietary adjustments are necessary to reduce ammonia levels and support biosecurity measures.

Nutritional Biosecurity Measures
Poultry immunity depends on proper nutrition, as essential nutrients regulate immune cell activity and function. Balanced diets rich in proteins, vitamins, trace minerals, and energy sources are critical for disease resistance. Probiotics enhance immunocompetence by stimulating antibody production, while prebiotics selectively promote beneficial gut bacteria, improving immune function. Additionally, mycotoxins in poultry feed suppress immune responses, making birds more susceptible to infections. Strict feed quality control and mycotoxin mitigation strategies should be integral to biosecurity programs.

Hygienic Disposal of Poultry Waste
Poultry operations generate waste, including dead birds, broken eggs, manure, litter, and contaminated equipment, which serve as reservoirs for pathogens. Proper disposal methods include burial, incineration, rendering, and composting.
Burial is effective but requires a 90-day period for pathogen deactivation before use as fertilizer. Incineration is reliable but often limited by facility size. Open burning is costly and environmentally unfavourable. Rendering is viable if decontamination is ensured, though private facilities may be reluctant to handle infected material. Composting within farm premises minimizes the risk of disease transmission during transport. Additionally, high-risk practices like using contaminated water and recycling untreated poultry waste should be strictly prohibited.

Wild Bird and Vector Control for Disease Prevention
Wild birds, particularly waterfowl, act as reservoirs for avian influenza and other pathogens, and play an important role in introducing infections to poultry farms. Effective biosecurity includes wild bird-proofing quarantine facilities and preventing their access to contaminated areas. Rodent control is equally essential, as rats and mice serve as mechanical carriers of the pathogens. A structured eradication program should minimize their dispersal from infected sites. Flying insects also contribute to disease transmission; thus, integrated pest management strategies should be implemented to reduce their presence in poultry sheds.
Immunomodulation through Nutritional Supplementation and Genetic Strategies
Regular supplementation of vitamins, minerals, and proteins strengthen poultry immunity and should be a core component of modern biosecurity. Nutrient deficiencies compromise resistance, increasing vulnerability to avian influenza and other diseases. As the influenza virus rapidly mutates and can exist as various subtypes and pathotypes, it questions the efficacy of existing vaccines and antivirals, and hence, genetic interventions offer a promising alternative. Screening poultry populations for disease-resistant genes, particularly in native breeds, and incorporating these traits through selective breeding can enhance flock resilience against infections.

Vaccination Strategies for Avian Influenza
Vaccination integrated with biosecurity measures can act as a critical tool for influenza control. Vaccines should provide adequate protection and minimize virus shedding. Vaccination programs coupled with virological and serological surveillance can be used to effectively detect viral mutations and assess vaccine effectiveness. In past influenza outbreaks in Maharashtra, Gujarat, and Madhya Pradesh, India successfully controlled the disease through culling and biosecurity measures. Establishing vaccine banks and enhancing domestic vaccine production are essential for rapid response to outbreaks. Policymakers must decide on vaccination strategies based on epidemiological data and national disease trends.

Strengthening Quarantine and Flock Profiling
Strict quarantine protocols are crucial in preventing disease introduction through newly acquired birds. Newly introduced poultry should be isolated for at least 21 days, monitored for clinical symptoms, and tested (blood, faecal, and nasal swabs) before integration with existing flocks. Beyond farm-level quarantine, strict regulations should be enforced to control cross-border movement of live poultry and poultry products.

Conclusion:
Effective biosecurity is the cornerstone of bird flu prevention and control, serving as the primary defence against disease outbreaks in poultry. Raising awareness among poultry farmers, industry stakeholders, and policymakers is essential for strengthening biosecurity at all levels. Training programs for grassroots poultry managers should be prioritized to ensure the proper implementation of preventive measures. In addition to immunity-boosting strategies and advancements in disease control, continuous surveillance of avian influenza and other infectious diseases is crucial. A proactive and well-enforced biosecurity framework not only safeguards poultry health and industry stability but also minimizes public health risks associated with zoonotic disease transmission. By integrating stringent biosecurity protocols with modern disease prevention strategies, the poultry sector can achieve long-term sustainability and resilience against emerging threats like avian influenza.

Dr. Anvesha Bhan1, Dr. Sundus Gazal2 and Dr. Sabahat Gazal3
Division of Veterinary Microbiology and Immunology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu

The poultry sector is among the fastest-growing industries, playing a crucial role in providing employment, income, and animal protein to both urban and rural populations, while also serving as manure for crops. Despite the global increase in meat supply, challenges such as bird handling, housing, rearing, and disease control still hinder the industry’s progress. During the monsoon season, continuous rainfall can lead to higher relative humidity and lower temperatures, affecting both the quality and quantity of feed. Additionally, wind speed can influence disease outbreaks. These weather changes impact poultry production, particularly for laying birds, as egg production declines in extremely cold or hot weather. Such conditions stress the birds, compromising their immune systems and reducing their disease resistance. Some of the common poultry diseases during the rainy season are:

Fowl Pox: Fowl pox is a highly contagious disease affecting poultry birds of all ages, caused by a poxvirus transmitted mainly by mosquitoes and other blood-sucking insects. The prevalence of fowl pox increases during the wet season due to the abundance of stagnant water, which provides breeding grounds for mosquitoes. Additionally, wet litter from poorly shielded poultry houses can lead to fly problems. Fowl pox exhibits round lesions with scabby centers on the birds’ skin, primarily on the wattle, face, comb, and occasionally on the legs. It can also affect the mouth and windpipe, causing lesions that may block the throat and lead to suffocation. Lesions on the face can spread to the eyes, potentially causing temporary or permanent blindness.

Fowl Cholera: Fowl cholera is a bacterial disease caused by Pasteurella multocida, affecting birds aged 6 weeks and above. It is highly contagious with high mortality in acute cases. The bacterium spreads readily during the rainy season as wet litter harbors numerous microorganisms.In acute cases, birds may die suddenly without prior signs, while chronic cases show symptoms similar to fowl typhoid, including yellow, green, or grey diarrhea; loss of appetite; labored breathing; drooped wings and tail feathers; ruffled feathers; swelling of leg joints, sinuses, wattles, and footpads.

Salmonellosis, Colibacillosis, Pullorum Disease (Bacillary White Diarrhea): These bacterial diseases affect birds of all ages and thrive in farms with poor sanitation, especially when wet litter is left unchecked. They impact the digestive system, presenting symptoms such as severe diarrhea, loss of appetite, depression and emaciation, chicks suffering from omphalitis, white pasty diarrhea in pullorum disease, huddling together and labored breathing.

Aspergillosis: Aspergillosis, caused by Aspergillus fumigatus, is prevalent during the rainy season due to high humidity, which dampens feed and litter, creating a conducive environment for fungal growth. Inhalation of Aspergillus spores lead to respiratory issues and lesions in the lungs. It is exhibited as Acute form which is common in young chicks and is characterized by rapid onset and high mortality with symptoms like lethargy, depression, loss of appetite, difficulty breathing, and cyanosis; or as Chronic form which develops subtly over weeks or months and affects older birds with symptoms like weight loss, reduced appetite, respiratory issues, and changes in vocalization.

Coccidiosis: Coccidiosis, a parasitic disease caused by the protozoan Eimeria spp. is an intestinal infection which causes extensive intestinal damage. It is widespread in poultry and game birds during the rainy season where wet litter and high pen temperatures favour the sporulation of oocysts of the parasite. Clinical signs include bloody faeces, ruffled feathers, anaemia, somnolence, severe diarrhoea, and high mortality. Decreased growth, feed and water consumption, weight loss, and decreased egg production are common. Infected survivors may suffer long-term performance loss.

Managemental Practices in Monsoons: Achieving Maximum Efficiency
The monsoon season brings challenges such as high relative humidity and temperature fluctuations. These extreme weather conditions create a favourable environment for the propagation of various pathogenic organisms, including bacteria, viruses, fungi, parasites, and vectors like flies and mosquitoes. This necessitates careful consideration and appropriate measures to optimize bird health and ensure efficient production.

Housing Management for Poultry During Monsoon:
A well-maintained shed is crucial for minimizing climatic stress and health challenges in poultry. Before the monsoon season, it is important to inspect the roof and walls for any holes or leaks and repair them promptly. Ensure the drainage ditch around the shed is clear to prevent waterlogging. The roof should have side overhangs of at least 3 to 4 feet to prevent rainwater from entering the shed. Cover the side walls of the empty shed with polythene curtains that are in good condition and can be adjusted based on ammonia concentration or rain intensity. Improper curtain management can lead to poor ventilation, resulting in ammonia buildup, which can cause issues such as improper digestion, abnormal respiration, and a high incidence of ascites. During the day, allow 1-2 feet opening at the top of the side curtains to ventilate ammonia and other undesirable gases. Atleast a 10-feet perimeter outside the shed should be kept clean and free of bushes and grasses. Waterlogging in the surrounding area can lead to propagation of insects like mosquitoes and flies inside the shed and since these act as vectors for many infectious diseases, proper cleanliness and pest control becomes crucial. To control the insect population regular spray of insecticides like bleaching powder and formalin (3-5%) should be done.

Litter Management in Poultry Housing During Monsoon:
A good litter material absorbs moisture when the surface is moist and the air is humid, and releases moisture when the air is dry. Ideally, the litter moisture content should be between 25% and 30%. If moisture falls to around 20%, the litter becomes too dusty, and if it rises to around 40%, the litter becomes wet and caked, which is undesirable. There are various issues that are faced with poor litter management viz., wet and caked litter promotes rapid microbial growth, which may cause infections leading to irritation, cracking, and infection of the foot. High moisture content in litter leads to ammonia buildup in the poultry house. Ammonia and other noxious gases can damage the respiratory tract lining, exposing birds to infections. Although the maximum permissible level of ammonia in the litter is 25 ppm, but adverse reactions including irritation of the eyes and respiratory tract start appearing at concentrations as low as 6 ppm, while reduced animal performance may be observed at 11 ppm. Thus, the level of ammonia in the shed must be kept at the minimum.

1. Moisture Control:
– Regularly check litter moisture. Compress the litter sample in hand; if it shows crevices and gently falls apart, moisture is optimal. If it forms a cohesive ball, it is too wet. If it crumbles easily, it is too dry.
– If litter moisture exceeds 40%, it indicates wet and caked litter which requires immediate disposal and replacement with fresh litter.
– Practice litter racking twice a day to prevent caking.
– To reduce litter moisture, add 1 kg of slaked lime and 150 gm of bleaching powder per 100 ft² of floor area.
– Operate ceiling fans at a ratio of one fan per 300 birds in deep litter broiler farms.

2. Overall Maintenance:
– To prevent mold growth, treat new litter with a 2% aqueous solution of copper sulphate spray.
– Regularly inspect and maintain the poultry house roof and walls to prevent leaks and ensure good drainage around the shed.
– Use polythene curtains to cover side walls and adjust them based on ammonia concentration and rain intensity, allowing for proper ventilation.
– Maintain cleanliness around the shed, keeping at least a 10-foot perimeter free from bushes and grasses to prevent waterlogging and insect breeding.
– Use insecticides, bleaching powder, and formalin spray (3-5%) outside the shed to control insect populations.

Feed and Water management to navigate through the Monsoon Season

Feed Management:
1. Adjust diet formulations to include all vital nutrients, considering the reduced feed intake of the birds due to high temperature and humidity.
2. Avoid long-term storage of feed as shelf life is shorter due to high humidity.
3. Prevent feed from heating up or forming lumps, which indicate decomposition and mold growth.
4. Ensure that vehicles for feed transport are leak-proof and maintain a 4-5 day extra feed stock to avoid frequent transportation during rainy days.
5. Use a Dunnage system to store feed bags. Stack bags on wooden or bamboo pallets at least 1 foot off the floor and away from side walls to avoid moisture contact and allow air circulation.
6. Implement a FIFO (First In, First Out) system for feed distribution.
7. Avoid wooden feed troughs to prevent mold growth and toxin production. Use plastic troughs for easier cleaning and disinfection.
8. Clean the feeders daily with a dry cloth.

Water Management:
1. Ensure clean, safe water supply as it significantly impacts flock performance.
2. Regularly sanitize water to prevent contamination, especially during the rainy season when E. coli and other coliform counts are higher.
3. Use water sanitizers with sufficient contact time and proper dosing.
4. Acidify drinking water to lower the pH, which reduces bacterial growth. Drinking water pH should preferably be around 5.0 to 5.5 to inhibit most pathogens. Poultry prefer water with a pH of 6 to 6.8.
5. Clean drinkers daily with detergents and bleaching powder to reduce water-borne diseases.
6. Clean pipelines at least once a week to reduce biofilm formation.
7. Monitor Oxidation-Reduction Potential (ORP) to evaluate the effectiveness of water sanitizers. An ORP value > 650 mV indicates good quality water, which can be effectively sanitized with 2-4 ppm free chlorine.